Transformation of eEF1BÎ́ into heat-shock response transcription factor by alternative splicing

Taku Kaitsuka, Kazuhito Tomizawa, Masayuki Matsushita

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Protein translation factors have crucial roles in a variety of stress responses. Here, we show that eukaryotic elongation factor 1B (eEF1BÎ́) changes its structure and function from a translation factor into a heat-shock response transcription factor by alternative splicing. The long isoform of eEF1BÎ́ (eEF1BL) is localized in the nucleus and induces heat-shock element (HSE)-containing genes in cooperation with heat-shock transcription factor 1 (HSF1). Moreover, the amino-terminal domain of eEF1BÎ́ L binds to NF-E2-related factor 2 (Nrf2) and induces stress response haem oxygenase 1 (HO1). Specific inhibition of eEF1B L with small-interfering RNA completely inhibits Nrf2-dependent HO1 induction. In addition, eEF1BÎ́ L directly binds to HSE oligo DNA in vitro and associates with the HSE consensus in the HO1 promoter region in vivo. Thus, the transcriptional role of eEF1BL could provide new insights into the molecular mechanism of stress responses.

Original languageEnglish
Pages (from-to)673-681
Number of pages9
JournalEMBO Reports
Volume12
Issue number7
DOIs
Publication statusPublished - Jul 1 2011
Externally publishedYes

Fingerprint

Peptide Elongation Factors
Heat-Shock Response
Alternative Splicing
Transcription Factors
Heme Oxygenase (Decyclizing)
NF-E2-Related Factor 2
Shock
Hot Temperature
Protein Biosynthesis
Genetic Promoter Regions
Small Interfering RNA
Protein Isoforms
Genes
heat shock transcription factor
DNA
Proteins

Keywords

  • eEF1B
  • heat shock
  • splicing

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

Cite this

Transformation of eEF1BÎ́ into heat-shock response transcription factor by alternative splicing. / Kaitsuka, Taku; Tomizawa, Kazuhito; Matsushita, Masayuki.

In: EMBO Reports, Vol. 12, No. 7, 01.07.2011, p. 673-681.

Research output: Contribution to journalArticle

Kaitsuka, Taku ; Tomizawa, Kazuhito ; Matsushita, Masayuki. / Transformation of eEF1BÎ́ into heat-shock response transcription factor by alternative splicing. In: EMBO Reports. 2011 ; Vol. 12, No. 7. pp. 673-681.
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