Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens

Yang Dong Guo, Hisano Hisano, Yoshiya Shimamoto, Toshihiko Yamada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. × Festuca pratensis Huds., 2n = 4x = 28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgene was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n = 2x = 14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume96
Issue number2
DOIs
Publication statusPublished - Feb 2009
Externally publishedYes

Keywords

  • Androgenic callus
  • Anther culture
  • Dihaploid
  • GUS activity
  • Transgenic forage grass

ASJC Scopus subject areas

  • Horticulture

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