Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens

Yang Dong Guo, Hiroshi Hisano, Yoshiya Shimamoto, Toshihiko Yamada

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Genetic transformation of androgenic-derived amphidiploid Festulolium plants (Lolium perenne L. × Festuca pratensis Huds., 2n = 4x = 28) by Agrobacterium tumefaciens has been achieved. Anther culture-induced calli of Festulolium "Bx351" were inoculated with Agrobacterium tumefaciens strain LBA4404 carrying pIG121-Hm encoding the hygromycin resistance (hph) and β-glucuronidase (uidA) genes under the control of a CaMV 35S promoter. Twenty-three putative transformants were obtained from the hygromycin selection, 19 of which (82.6%) showed GUS activity. The integration of transgene was detected by using genomic DNA PCR analysis, RT-PCR analysis and Southern blot hybridization, respectively, which revealed that foreign gene was integrated into the genomes of dihaploid transformants (2n = 2x = 14). The haploid embryogenic system offers a stable means of transformation, as the introduced trait can be readily fixed through chromosome doubling.

Original languageEnglish
Pages (from-to)219-227
Number of pages9
JournalPlant Cell, Tissue and Organ Culture
Volume96
Issue number2
DOIs
Publication statusPublished - Feb 2009
Externally publishedYes

Fingerprint

Festulolium
Festuca pratensis
Agrobacterium radiobacter
Lolium perenne
dihaploidy
anther culture
genetic transformation
haploidy
Southern blotting
transgenes
callus
hybridization
genes
reverse transcriptase polymerase chain reaction
promoter regions
chromosomes
genomics
genome
DNA

Keywords

  • Androgenic callus
  • Anther culture
  • Dihaploid
  • GUS activity
  • Transgenic forage grass

ASJC Scopus subject areas

  • Horticulture

Cite this

Transformation of androgenic-derived Festulolium plants (Lolium perenne L. × Festuca pratensis Huds.) by Agrobacterium tumefaciens. / Guo, Yang Dong; Hisano, Hiroshi; Shimamoto, Yoshiya; Yamada, Toshihiko.

In: Plant Cell, Tissue and Organ Culture, Vol. 96, No. 2, 02.2009, p. 219-227.

Research output: Contribution to journalArticle

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