Transdifferentiation of retinal pigment epithelial cells into lens in suspension culture

Y. Tsutsui, Toshihiko Matsuo, N. Matsuo

Research output: Contribution to journalArticle

Abstract

Purpose. To elucidate factors influencing transdifferentiation of retinal pigment epithelial (RPE) cells in suspension culture. Methods. RPE eel Is were isolated as a sheet from 7.5-day-old chick embryos and cultured either as a sheet or dissociated eelIs after trypsin treatment in a non-adherent dish for suspension culture for one month. Basic fibroblast growth factor (bFGF) was added to the medium at varying concentrations of 0-200 ng/ml. Results. At the higher concentrations (10 or 200 ng/ml) of bFGF, a rounded-up RPE sheet grew to develop a nonpigmented lump with an onion-like structure, while dissociated RPE cells, although they reaggregated, did not form such structure. Histologically, the onion-like structure consisted of lens-fiber-like cells, positive for ant i-crystall in antibody and anti-bFGF-receptor antibody by immunohistochemistry. The formation of this lens-1ike structure did not occur at the concentration of bFGF, 1 ng/ml or lower. Conclusion. The transdifferentiation of RPE cells into lens in suspension culture was dependent upon the concentration of bFGF as well as proper three-dimensional interaction among each RPE cell.

Original languageEnglish
JournalInvestigative Ophthalmology and Visual Science
Volume38
Issue number4
Publication statusPublished - 1997

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Retinal Pigments
Lenses
Fibroblast Growth Factor 2
Suspensions
Epithelial Cells
Onions
Fibroblast Growth Factor 1
Fibroblast Growth Factor Receptors
Eels
Ants
Chick Embryo
Trypsin
Anti-Idiotypic Antibodies
Immunohistochemistry
Antibodies

ASJC Scopus subject areas

  • Ophthalmology

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Transdifferentiation of retinal pigment epithelial cells into lens in suspension culture. / Tsutsui, Y.; Matsuo, Toshihiko; Matsuo, N.

In: Investigative Ophthalmology and Visual Science, Vol. 38, No. 4, 1997.

Research output: Contribution to journalArticle

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AB - Purpose. To elucidate factors influencing transdifferentiation of retinal pigment epithelial (RPE) cells in suspension culture. Methods. RPE eel Is were isolated as a sheet from 7.5-day-old chick embryos and cultured either as a sheet or dissociated eelIs after trypsin treatment in a non-adherent dish for suspension culture for one month. Basic fibroblast growth factor (bFGF) was added to the medium at varying concentrations of 0-200 ng/ml. Results. At the higher concentrations (10 or 200 ng/ml) of bFGF, a rounded-up RPE sheet grew to develop a nonpigmented lump with an onion-like structure, while dissociated RPE cells, although they reaggregated, did not form such structure. Histologically, the onion-like structure consisted of lens-fiber-like cells, positive for ant i-crystall in antibody and anti-bFGF-receptor antibody by immunohistochemistry. The formation of this lens-1ike structure did not occur at the concentration of bFGF, 1 ng/ml or lower. Conclusion. The transdifferentiation of RPE cells into lens in suspension culture was dependent upon the concentration of bFGF as well as proper three-dimensional interaction among each RPE cell.

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