Transcriptomic comparison between beetle strains selected for short and long durations of death feigning

Hironobu Uchiyama, Ken Sasaki, Shogo Hinosawa, Keisuke Tanaka, Kentarou Matsumura, Shunsuke Yajima, Takahisa Miyatake

Research output: Contribution to journalArticle

Abstract

The molecular basis of death feigning, an antipredator behavior that has received much attention recently, was analyzed. We compared the gene expression profiles of strains with different behaviors, i.e., different durations of death feigning, in the beetle Tribolium castaneum. Beetles artificially selected for short (S) and long (L) durations of death feigning for many generations were compared thoroughly by RNA sequencing. We identified 518 differentially expressed genes (DEGs) between the strains. The strains also showed divergence in unexpected gene expression regions. As expected from previous physiological studies, genes associated with the metabolic pathways of tyrosine, a precursor of dopamine, were differentially expressed between the S and L strains; these enzyme-encoding genes were expressed at higher levels in the L strain than in the S strain. We also found that several genes associated with insulin signaling were expressed at higher levels in the S strain than in the L strain. Quantitative real-time PCR analysis showed that the relative expression levels of Tchpd (encoding 4-hydroxyphenylpyruvate dioxygenase, Hpd) and Tcnat (encoding N-acetyltransferase, Nat) were significantly higher in the L strain than in the S strain, suggesting the influence of these enzymes on the supply of dopamine and duration of death feigning.

Original languageEnglish
Article number14001
JournalScientific reports
Volume9
Issue number1
DOIs
Publication statusPublished - Dec 1 2019

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Tonic Immobility Response
Beetles
Genes
4-Hydroxyphenylpyruvate Dioxygenase
Dopamine
Tribolium
RNA Sequence Analysis
Acetyltransferases
Enzymes
Metabolic Networks and Pathways
Transcriptome
Tyrosine
Real-Time Polymerase Chain Reaction
Insulin
Gene Expression

ASJC Scopus subject areas

  • General

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Transcriptomic comparison between beetle strains selected for short and long durations of death feigning. / Uchiyama, Hironobu; Sasaki, Ken; Hinosawa, Shogo; Tanaka, Keisuke; Matsumura, Kentarou; Yajima, Shunsuke; Miyatake, Takahisa.

In: Scientific reports, Vol. 9, No. 1, 14001, 01.12.2019.

Research output: Contribution to journalArticle

Uchiyama, Hironobu ; Sasaki, Ken ; Hinosawa, Shogo ; Tanaka, Keisuke ; Matsumura, Kentarou ; Yajima, Shunsuke ; Miyatake, Takahisa. / Transcriptomic comparison between beetle strains selected for short and long durations of death feigning. In: Scientific reports. 2019 ; Vol. 9, No. 1.
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