Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles

Kogen Okita, Engkong Tan, Hina Satone, Shigeharu Kinoshita, Shuichi Asakawa, Daisuke Ojima, Hideki Yamazaki, Kazutaka Sakiyama, Tomohiro Takatani, Osamu Arakawa, Atsushi Hagiwara, Yoshitaka Sakakura

Research output: Contribution to journalArticle

Abstract

To reveal the sensing of tetrodotoxin (TTX) by tiger puffer Takifugu rubripes juveniles and its action in the central nervous system (CNS), we conducted transcriptome analysis using next-generation sequencing for the olfactory system and brain of non-toxic cultured juveniles administered TTX. Sixty-seven million reads from the nasal region (olfactory epithelium and skin) and the brain of each of three individuals of the control, TTX-sensing and TTX-administered juveniles were assembled into 153,958 contigs. Mapping raw reads from each sample onto the nucleotide sequences of predicted transcripts in the T. rubripes genome (FUGU version 4) and the de novo assembled contigs to investigate their frequency of expression revealed that the expression of 21 and 81 known genes significantly changed in TTX-sensing and TTX-administered juveniles in comparison with control juveniles, respectively. These genes included those related to feeding regulation and a reward system, and indicated that TTX ingestion of T. rubripes juveniles is controlled in the feeding center in the brain, that T. rubripes may sense TTX as a reward, and that accumulated TTX directly acts on the central nervous system to adjust TTX ingestion.

Original languageEnglish
Pages (from-to)401-412
Number of pages12
JournalFisheries Science
Volume83
Issue number3
DOIs
Publication statusPublished - May 1 2017
Externally publishedYes

Fingerprint

Takifugu rubripes
tetrodotoxin
nervous system
transcriptomics
central nervous system
brain
gene
analysis
ingestion
skin
genome
skin (animal)
epithelium
genes

Keywords

  • Central nervous system
  • Feeding center
  • Reward system
  • RNA sequencing
  • Takifugu rubripes
  • Tetrodotoxin (TTX)

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles. / Okita, Kogen; Tan, Engkong; Satone, Hina; Kinoshita, Shigeharu; Asakawa, Shuichi; Ojima, Daisuke; Yamazaki, Hideki; Sakiyama, Kazutaka; Takatani, Tomohiro; Arakawa, Osamu; Hagiwara, Atsushi; Sakakura, Yoshitaka.

In: Fisheries Science, Vol. 83, No. 3, 01.05.2017, p. 401-412.

Research output: Contribution to journalArticle

Okita, K, Tan, E, Satone, H, Kinoshita, S, Asakawa, S, Ojima, D, Yamazaki, H, Sakiyama, K, Takatani, T, Arakawa, O, Hagiwara, A & Sakakura, Y 2017, 'Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles', Fisheries Science, vol. 83, no. 3, pp. 401-412. https://doi.org/10.1007/s12562-017-1079-z
Okita, Kogen ; Tan, Engkong ; Satone, Hina ; Kinoshita, Shigeharu ; Asakawa, Shuichi ; Ojima, Daisuke ; Yamazaki, Hideki ; Sakiyama, Kazutaka ; Takatani, Tomohiro ; Arakawa, Osamu ; Hagiwara, Atsushi ; Sakakura, Yoshitaka. / Transcriptome analysis of tetrodotoxin sensing and tetrodotoxin action in the central nervous system of tiger puffer Takifugu rubripes juveniles. In: Fisheries Science. 2017 ; Vol. 83, No. 3. pp. 401-412.
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