Transcriptome analysis of barley identifies heat shock and HD-Zip I transcription factors up-regulated in response to multiple abiotic stresses

Takashi Matsumoto, Hiromi Morishige, Tsuyoshi Tanaka, Hiroyuki Kanamori, Takao Komatsuda, Kazuhiro Sato, Takeshi Itoh, Jianzhong Wu, Shingo Nakamura

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Analyzing barley gene expression profiles in response to abiotic stress is critical to understanding how barley manages stress, and provides vital information to improve environmental stress tolerance for stable crop production. We developed an Agilent 60-mer oligo DNA microarray with 42,491 probe sets based on the sequences of 36,632 barley (Hordeum vulgare L.) full-length cDNA clones and conducted global expression profiling on barley seedlings subjected to desiccation, salt, cold and abscisic acid (ABA). We identified 281 genes that were differentially expressed in response to desiccation, salt, and cold stresses and ABA treatment. Among them, a class C heat shock transcription factor (HvHsfC1) and a homeodomain leucine zipper (HD-Zip) family I transcription factor (HvHox22) showed more than tenfold and fourfold higher expression, respectively, in response to the stimuli. Heat shock and HD-Zip transcription factors function as important regulators in stress responses in rice (Oryza sativa) and Arabidopsis thaliana; our results suggest these two transcription factors also play important roles in abiotic stress responses in barley. We mapped HvHox22 to the long arm of chromosome 2H and HvHsfC1 to the long arm of 4H, where drought resistance quantitative trait loci were previously detected. Our microarray data and identification of these stress response genes provide key information for dissection of the mechanism of abiotic stress tolerance in barley.

Original languageEnglish
Pages (from-to)1-8
Number of pages8
JournalMolecular Breeding
DOIs
Publication statusAccepted/In press - 2014

Fingerprint

Leucine Zippers
leucine zipper
Gene Expression Profiling
Hordeum
transcriptomics
abiotic stress
heat stress
Shock
Transcription Factors
transcription factors
Hot Temperature
barley
stress response
Desiccation
desiccation (plant physiology)
Abscisic Acid
stress tolerance
abscisic acid
Salts
cold stress

Keywords

  • Barley
  • Drought tolerance
  • Full-length cDNA
  • Stress response
  • Transcription factors
  • Transcriptome

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Biotechnology
  • Plant Science
  • Agronomy and Crop Science

Cite this

Transcriptome analysis of barley identifies heat shock and HD-Zip I transcription factors up-regulated in response to multiple abiotic stresses. / Matsumoto, Takashi; Morishige, Hiromi; Tanaka, Tsuyoshi; Kanamori, Hiroyuki; Komatsuda, Takao; Sato, Kazuhiro; Itoh, Takeshi; Wu, Jianzhong; Nakamura, Shingo.

In: Molecular Breeding, 2014, p. 1-8.

Research output: Contribution to journalArticle

Matsumoto, Takashi ; Morishige, Hiromi ; Tanaka, Tsuyoshi ; Kanamori, Hiroyuki ; Komatsuda, Takao ; Sato, Kazuhiro ; Itoh, Takeshi ; Wu, Jianzhong ; Nakamura, Shingo. / Transcriptome analysis of barley identifies heat shock and HD-Zip I transcription factors up-regulated in response to multiple abiotic stresses. In: Molecular Breeding. 2014 ; pp. 1-8.
@article{32ec631f3c7041e49e80b812374c7968,
title = "Transcriptome analysis of barley identifies heat shock and HD-Zip I transcription factors up-regulated in response to multiple abiotic stresses",
abstract = "Analyzing barley gene expression profiles in response to abiotic stress is critical to understanding how barley manages stress, and provides vital information to improve environmental stress tolerance for stable crop production. We developed an Agilent 60-mer oligo DNA microarray with 42,491 probe sets based on the sequences of 36,632 barley (Hordeum vulgare L.) full-length cDNA clones and conducted global expression profiling on barley seedlings subjected to desiccation, salt, cold and abscisic acid (ABA). We identified 281 genes that were differentially expressed in response to desiccation, salt, and cold stresses and ABA treatment. Among them, a class C heat shock transcription factor (HvHsfC1) and a homeodomain leucine zipper (HD-Zip) family I transcription factor (HvHox22) showed more than tenfold and fourfold higher expression, respectively, in response to the stimuli. Heat shock and HD-Zip transcription factors function as important regulators in stress responses in rice (Oryza sativa) and Arabidopsis thaliana; our results suggest these two transcription factors also play important roles in abiotic stress responses in barley. We mapped HvHox22 to the long arm of chromosome 2H and HvHsfC1 to the long arm of 4H, where drought resistance quantitative trait loci were previously detected. Our microarray data and identification of these stress response genes provide key information for dissection of the mechanism of abiotic stress tolerance in barley.",
keywords = "Barley, Drought tolerance, Full-length cDNA, Stress response, Transcription factors, Transcriptome",
author = "Takashi Matsumoto and Hiromi Morishige and Tsuyoshi Tanaka and Hiroyuki Kanamori and Takao Komatsuda and Kazuhiro Sato and Takeshi Itoh and Jianzhong Wu and Shingo Nakamura",
year = "2014",
doi = "10.1007/s11032-014-0048-9",
language = "English",
pages = "1--8",
journal = "Molecular Breeding",
issn = "1380-3743",
publisher = "Springer Netherlands",

}

TY - JOUR

T1 - Transcriptome analysis of barley identifies heat shock and HD-Zip I transcription factors up-regulated in response to multiple abiotic stresses

AU - Matsumoto, Takashi

AU - Morishige, Hiromi

AU - Tanaka, Tsuyoshi

AU - Kanamori, Hiroyuki

AU - Komatsuda, Takao

AU - Sato, Kazuhiro

AU - Itoh, Takeshi

AU - Wu, Jianzhong

AU - Nakamura, Shingo

PY - 2014

Y1 - 2014

N2 - Analyzing barley gene expression profiles in response to abiotic stress is critical to understanding how barley manages stress, and provides vital information to improve environmental stress tolerance for stable crop production. We developed an Agilent 60-mer oligo DNA microarray with 42,491 probe sets based on the sequences of 36,632 barley (Hordeum vulgare L.) full-length cDNA clones and conducted global expression profiling on barley seedlings subjected to desiccation, salt, cold and abscisic acid (ABA). We identified 281 genes that were differentially expressed in response to desiccation, salt, and cold stresses and ABA treatment. Among them, a class C heat shock transcription factor (HvHsfC1) and a homeodomain leucine zipper (HD-Zip) family I transcription factor (HvHox22) showed more than tenfold and fourfold higher expression, respectively, in response to the stimuli. Heat shock and HD-Zip transcription factors function as important regulators in stress responses in rice (Oryza sativa) and Arabidopsis thaliana; our results suggest these two transcription factors also play important roles in abiotic stress responses in barley. We mapped HvHox22 to the long arm of chromosome 2H and HvHsfC1 to the long arm of 4H, where drought resistance quantitative trait loci were previously detected. Our microarray data and identification of these stress response genes provide key information for dissection of the mechanism of abiotic stress tolerance in barley.

AB - Analyzing barley gene expression profiles in response to abiotic stress is critical to understanding how barley manages stress, and provides vital information to improve environmental stress tolerance for stable crop production. We developed an Agilent 60-mer oligo DNA microarray with 42,491 probe sets based on the sequences of 36,632 barley (Hordeum vulgare L.) full-length cDNA clones and conducted global expression profiling on barley seedlings subjected to desiccation, salt, cold and abscisic acid (ABA). We identified 281 genes that were differentially expressed in response to desiccation, salt, and cold stresses and ABA treatment. Among them, a class C heat shock transcription factor (HvHsfC1) and a homeodomain leucine zipper (HD-Zip) family I transcription factor (HvHox22) showed more than tenfold and fourfold higher expression, respectively, in response to the stimuli. Heat shock and HD-Zip transcription factors function as important regulators in stress responses in rice (Oryza sativa) and Arabidopsis thaliana; our results suggest these two transcription factors also play important roles in abiotic stress responses in barley. We mapped HvHox22 to the long arm of chromosome 2H and HvHsfC1 to the long arm of 4H, where drought resistance quantitative trait loci were previously detected. Our microarray data and identification of these stress response genes provide key information for dissection of the mechanism of abiotic stress tolerance in barley.

KW - Barley

KW - Drought tolerance

KW - Full-length cDNA

KW - Stress response

KW - Transcription factors

KW - Transcriptome

UR - http://www.scopus.com/inward/record.url?scp=84893644405&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893644405&partnerID=8YFLogxK

U2 - 10.1007/s11032-014-0048-9

DO - 10.1007/s11032-014-0048-9

M3 - Article

AN - SCOPUS:84893644405

SP - 1

EP - 8

JO - Molecular Breeding

JF - Molecular Breeding

SN - 1380-3743

ER -