Transcriptome analysis and identification of a transcriptional regulatory network in the response to H2O2

Ayaka Hieno, Hushna Ara Naznin, Keiko Inaba-Hasegawa, Tomoko Yokogawa, Natsuki Hayami, Mika Nomoto, Yasuomi Tada, Takashi Yokogawa, Mieko Higuchi-Takeuchi, Kosuke Hanada, Minami Matsui, Yoko Ikeda, Yuko Hojo, Takashi Hirayama, Kazutaka Kusunoki, Hiroyuki Koyama, Nobutaka Mitsuda, Yoshiharu Y. Yamamoto

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3 Citations (Scopus)

Abstract

Hydrogen peroxide (H2O2) is a common signal molecule initiating transcriptional responses to all the known biotic and abiotic stresses of land plants. However, the degree of involvement of H2O2 in these stress responses has not yet been well studied. Here we identify time-dependent transcriptome profiles stimulated by H2O2 application in Arabidopsis (Arabidopsis thaliana) seedlings. Promoter prediction based on transcriptome data suggests strong crosstalk among high light, heat, and wounding stress responses in terms of environmental stresses and between the abscisic acid (ABA) and salicylic acid (SA) responses in terms of phytohormone signaling. Quantitative analysis revealed that ABA accumulation is induced by H2O2 but SA is not, suggesting that the implied crosstalk with ABA is achieved through ABA accumulation while the crosstalk with SA is different. We identified potential direct regulatory pairs between regulator transcription factor (TF) proteins and their regulated TF genes based on the time-course transcriptome analysis for the H2O2 response, in vivo regulation of the regulated TF by the regulator TF identified by expression analysis of mutants and overexpressors, and in vitro binding of the regulator TF protein to the target TF promoter. These analyses enabled the establishment of part of the transcriptional regulatory network for the H2O2 response composed of 15 regulatory pairs of TFs, including five pairs previously reported. This regulatory network is suggested to be involved in a wide range of biotic and abiotic stress responses in Arabidopsis.

Original languageEnglish
Pages (from-to)1629-1646
Number of pages18
JournalPlant physiology
Volume180
Issue number3
DOIs
Publication statusPublished - Jul 2019

ASJC Scopus subject areas

  • Physiology
  • Genetics
  • Plant Science

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    Hieno, A., Naznin, H. A., Inaba-Hasegawa, K., Yokogawa, T., Hayami, N., Nomoto, M., Tada, Y., Yokogawa, T., Higuchi-Takeuchi, M., Hanada, K., Matsui, M., Ikeda, Y., Hojo, Y., Hirayama, T., Kusunoki, K., Koyama, H., Mitsuda, N., & Yamamoto, Y. Y. (2019). Transcriptome analysis and identification of a transcriptional regulatory network in the response to H2O2 Plant physiology, 180(3), 1629-1646. https://doi.org/10.1104/pp.18.01426