TY - JOUR
T1 - Transcriptome analysis and identification of a transcriptional regulatory network in the response to H2O2
AU - Hieno, Ayaka
AU - Naznin, Hushna Ara
AU - Inaba-Hasegawa, Keiko
AU - Yokogawa, Tomoko
AU - Hayami, Natsuki
AU - Nomoto, Mika
AU - Tada, Yasuomi
AU - Yokogawa, Takashi
AU - Higuchi-Takeuchi, Mieko
AU - Hanada, Kosuke
AU - Matsui, Minami
AU - Ikeda, Yoko
AU - Hojo, Yuko
AU - Hirayama, Takashi
AU - Kusunoki, Kazutaka
AU - Koyama, Hiroyuki
AU - Mitsuda, Nobutaka
AU - Yamamoto, Yoshiharu Y.
N1 - Funding Information:
1This work was supported in part by the Japan Science and Technology Agency, Advanced Low Carbon Technology Research and Development Program (to Y.Y.Y.), Grant-in-Aid for Scientific Research on Innovative Areas “Plant Perception” (grant no. 23120511 to Y.Y.Y.), and the Japan Advanced Plant Science Network. 2Author for contact: yyy@gifu-u.ac.jp. 3Senior author.
Funding Information:
This work was supported in part by the Japan Science and Technology Agency, Advanced Low Carbon Technology Research and Development Program (to Y.Y.Y.), Grant-in-Aid for Scientific Research on Innovative Areas ?Plant Perception? (grant no. 23120511 to Y.Y.Y.), and the Japan Advanced Plant Science Network. The authors appreciate Dr. Frank Van Breusegem and Dr. Inge De Clercq of Ghent University for critical reading of the article. We thank Dr. Masanori Okamoto of Utsunomiya University and Dr. Takashi Hirayama of Okayama University for kindly providing Arabidopsis mutant seeds, and also Dr. Mutsutomo Tokizawa and Mr. Daichi Obata in our laboratory for technical assistance with protein-DNA binding assays.
Publisher Copyright:
© 2019 American Society of Plant Biologists.
PY - 2019/7
Y1 - 2019/7
N2 - Hydrogen peroxide (H2O2) is a common signal molecule initiating transcriptional responses to all the known biotic and abiotic stresses of land plants. However, the degree of involvement of H2O2 in these stress responses has not yet been well studied. Here we identify time-dependent transcriptome profiles stimulated by H2O2 application in Arabidopsis (Arabidopsis thaliana) seedlings. Promoter prediction based on transcriptome data suggests strong crosstalk among high light, heat, and wounding stress responses in terms of environmental stresses and between the abscisic acid (ABA) and salicylic acid (SA) responses in terms of phytohormone signaling. Quantitative analysis revealed that ABA accumulation is induced by H2O2 but SA is not, suggesting that the implied crosstalk with ABA is achieved through ABA accumulation while the crosstalk with SA is different. We identified potential direct regulatory pairs between regulator transcription factor (TF) proteins and their regulated TF genes based on the time-course transcriptome analysis for the H2O2 response, in vivo regulation of the regulated TF by the regulator TF identified by expression analysis of mutants and overexpressors, and in vitro binding of the regulator TF protein to the target TF promoter. These analyses enabled the establishment of part of the transcriptional regulatory network for the H2O2 response composed of 15 regulatory pairs of TFs, including five pairs previously reported. This regulatory network is suggested to be involved in a wide range of biotic and abiotic stress responses in Arabidopsis.
AB - Hydrogen peroxide (H2O2) is a common signal molecule initiating transcriptional responses to all the known biotic and abiotic stresses of land plants. However, the degree of involvement of H2O2 in these stress responses has not yet been well studied. Here we identify time-dependent transcriptome profiles stimulated by H2O2 application in Arabidopsis (Arabidopsis thaliana) seedlings. Promoter prediction based on transcriptome data suggests strong crosstalk among high light, heat, and wounding stress responses in terms of environmental stresses and between the abscisic acid (ABA) and salicylic acid (SA) responses in terms of phytohormone signaling. Quantitative analysis revealed that ABA accumulation is induced by H2O2 but SA is not, suggesting that the implied crosstalk with ABA is achieved through ABA accumulation while the crosstalk with SA is different. We identified potential direct regulatory pairs between regulator transcription factor (TF) proteins and their regulated TF genes based on the time-course transcriptome analysis for the H2O2 response, in vivo regulation of the regulated TF by the regulator TF identified by expression analysis of mutants and overexpressors, and in vitro binding of the regulator TF protein to the target TF promoter. These analyses enabled the establishment of part of the transcriptional regulatory network for the H2O2 response composed of 15 regulatory pairs of TFs, including five pairs previously reported. This regulatory network is suggested to be involved in a wide range of biotic and abiotic stress responses in Arabidopsis.
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U2 - 10.1104/pp.18.01426
DO - 10.1104/pp.18.01426
M3 - Article
C2 - 31064811
AN - SCOPUS:85069238194
VL - 180
SP - 1629
EP - 1646
JO - Plant Physiology
JF - Plant Physiology
SN - 0032-0889
IS - 3
ER -