Transcriptional trans-repression by the c-myb proto-oncogene product

Hideki Nakagoshi, Takahiro Nagase, Yoshio Ueno, Shunsuke Ishii

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

We report that the c-myb protein binds to another site, MBS-l1, in the SV4O enhancer with low affinity. In co-transfection experiments with a c-myb expression plasmid, tandem repeats of the sequence containing the MBS-I1 site induced c-myb-dependent transcriptional repression. Results of mutational analyses of the sequence around the MBS-II site suggested that the c-myb protein represses transcription by competing with another trans-activator. These results indicate the c-myb protein can regulate transcription not only positively but also negatively.

Original languageEnglish
Pages (from-to)7315-7324
Number of pages10
JournalNucleic acids research.
Volume17
Issue number18
DOIs
Publication statusPublished - Sep 25 1989
Externally publishedYes

Fingerprint

Proto-Oncogene Proteins c-myb
Transcription
Proteins
Protein
Tandem Repeat Sequences
Trans-Activators
Affine transformation
Transfection
Sequence Analysis
Plasmids
Dependent
Experiment
Experiments

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Transcriptional trans-repression by the c-myb proto-oncogene product. / Nakagoshi, Hideki; Nagase, Takahiro; Ueno, Yoshio; Ishii, Shunsuke.

In: Nucleic acids research., Vol. 17, No. 18, 25.09.1989, p. 7315-7324.

Research output: Contribution to journalArticle

Nakagoshi, Hideki ; Nagase, Takahiro ; Ueno, Yoshio ; Ishii, Shunsuke. / Transcriptional trans-repression by the c-myb proto-oncogene product. In: Nucleic acids research. 1989 ; Vol. 17, No. 18. pp. 7315-7324.
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