Transcriptional responses of a bicarbonate-tolerant monocot, Puccinellia tenuiflora, And a related bicarbonate-sensitive species, Poa annua, To NaHCO3 stress

Shio Kobayashi, Hina Satone, Engkong Tan, Hiroyuki Kurokochi, Shuichi Asakawa, Shenkui Liu, Tetsuo Takano

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Puccinellia tenuiflora is an alkaline salt-tolerant monocot found in saline-alkali soil in China. To identify the genes which are determining the higher tolerance of P. tenuiflora compared to bicarbonate sensitive species, we examined the responses of P. tenuiflora and a related bicarbonate-sensitive Poeae plant, Poa annua, to two days of 20 mM NaHCO3 stress by RNA-seq analysis. We obtained 28 and 38 million reads for P. tenuiflora and P. annua, respectively. For each species, the reads of both unstressed and stressed samples were combined for de novo assembly of contigs. We obtained 77,329 contigs for P. tenuiflora and 115,335 contigs for P. annua. NaHCO3 stress resulted in greater than two-fold absolute expression value changes in 157 of the P. tenuiflora contigs and 1090 of P. annua contigs. Homologs of the genes involved in Fe acquisition, which are important for the survival of plants under alkaline stress, were up-regulated in P. tenuiflora and down-regulated in P. annua. The smaller number of the genes differentially regulated in P. tenuiflora suggests that the genes regulating bicarbonate tolerance are constitutively expressed in P. tenuiflora.

Original languageEnglish
Pages (from-to)496-509
Number of pages14
JournalInternational Journal of Molecular Sciences
Volume16
Issue number1
DOIs
Publication statusPublished - Dec 29 2015
Externally publishedYes

Fingerprint

Poa
Bicarbonates
genes
carbonates
Genes
Alkalies
RNA
China
alkalies
soils
acquisition
Soil
Salts
assembly
salts
Soils

Keywords

  • Alkaline salt stress
  • Bicarbonate
  • Poa annua
  • Puccinellia tenuiflora
  • RNA-seq

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Spectroscopy
  • Inorganic Chemistry
  • Catalysis
  • Molecular Biology
  • Computer Science Applications
  • Medicine(all)

Cite this

Transcriptional responses of a bicarbonate-tolerant monocot, Puccinellia tenuiflora, And a related bicarbonate-sensitive species, Poa annua, To NaHCO3 stress. / Kobayashi, Shio; Satone, Hina; Tan, Engkong; Kurokochi, Hiroyuki; Asakawa, Shuichi; Liu, Shenkui; Takano, Tetsuo.

In: International Journal of Molecular Sciences, Vol. 16, No. 1, 29.12.2015, p. 496-509.

Research output: Contribution to journalArticle

Kobayashi, Shio ; Satone, Hina ; Tan, Engkong ; Kurokochi, Hiroyuki ; Asakawa, Shuichi ; Liu, Shenkui ; Takano, Tetsuo. / Transcriptional responses of a bicarbonate-tolerant monocot, Puccinellia tenuiflora, And a related bicarbonate-sensitive species, Poa annua, To NaHCO3 stress. In: International Journal of Molecular Sciences. 2015 ; Vol. 16, No. 1. pp. 496-509.
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