Transcriptional repressor foxl1 regulates central nervous system development by suppressing shh expression in zebra fish

Chisako Nakada, Shinya Satoh, Yoko Tabata, Ken Ichi Arai, Sumiko Watanabe

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

We identified zebra fish forkhead transcription factor 11 (zfoxl1) as a gene strongly expressed in neural tissues such as midbrain, hindbrain, and the otic vesicle at the early embryonic stage. Loss of the function of zfoxl1 effected by morpholino antisense oligonucleotide resulted in defects in midbrain and eye development, and in that of formation of the pectoral fins. Interestingly, ectopic expression of shh in the midbrain and elevated pax2a expression in the optic stalk were observed in foxl1 MO-injected embryos. In contrast, expression of pax6a, which is negatively regulated by shh, was suppressed in the thalamus and pretectum regions, supporting the idea of augmentation of the shh signaling pathway by suppression of foxl1. Expression of zfoxl1-EnR (repressing) rather than zfoxl1-VP16 (activating) resulted in a phenotype similar to that induced by foxl1-mRNA, suggesting that foxl1 may act as a transcriptional repressor of shh in zebra fish embryos. Supporting this notion, foxl1 suppressed isolated 2.7-kh shh promoter activity in PC12 cells, and the minimal region of foxl1 required for its transcriptional repressor activity showed strong homology with the groucho binding motif, which is found in genes encoding various homeodomain proteins. In view of all of our data taken together, we propose zfoxl1 to be a novel regulator of neural development that acts by suppressing shh expression.

Original languageEnglish
Pages (from-to)7246-7257
Number of pages12
JournalMolecular and Cellular Biology
Volume26
Issue number19
DOIs
Publication statusPublished - Oct 2006
Externally publishedYes

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Zebrafish
Mesencephalon
Central Nervous System
Embryonic Structures
Forkhead Transcription Factors
Homeodomain Proteins
Morpholinos
Rhombencephalon
Antisense Oligonucleotides
PC12 Cells
Thalamus
Genes
Ear
Phenotype
Messenger RNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

Transcriptional repressor foxl1 regulates central nervous system development by suppressing shh expression in zebra fish. / Nakada, Chisako; Satoh, Shinya; Tabata, Yoko; Arai, Ken Ichi; Watanabe, Sumiko.

In: Molecular and Cellular Biology, Vol. 26, No. 19, 10.2006, p. 7246-7257.

Research output: Contribution to journalArticle

Nakada, Chisako ; Satoh, Shinya ; Tabata, Yoko ; Arai, Ken Ichi ; Watanabe, Sumiko. / Transcriptional repressor foxl1 regulates central nervous system development by suppressing shh expression in zebra fish. In: Molecular and Cellular Biology. 2006 ; Vol. 26, No. 19. pp. 7246-7257.
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