Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene

Masaki Mori, Masahiro Nakajima, Yasuo Mikami, Shoji Seki, Masaharu Takigawa, Toshikazu Kubo, Shiro Ikegawa

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

Cartilage intermediate layer protein (CILP) is an extracellular matrix protein abundant in cartilaginous tissues. CILP is implicated in common musculoskeletal disorders, including osteoarthritis and lumbar disc disease. Regulation of the CILP gene is largely unknown, however. We have found that CILP mRNA expression is induced by TGF-β1 and dependent upon signaling via TGF-β receptors. TGF-β1 induction of CILP is mediated by Smad3, which acts directly through cis-elements in the CILP promoter region. Pathways other than Smad3 also are involved in TGF-β1 induction of CILP. These observations, together with the finding that CILP protein binds and inhibits TGF-β1, suggest that CILP and TGF-β1 may form a functional feedback loop that controls chondrocyte metabolism.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume341
Issue number1
DOIs
Publication statusPublished - Mar 3 2006

Fingerprint

Cartilage
Proteins
Extracellular Matrix Proteins
Chondrocytes
Genetic Promoter Regions
Metabolism
Osteoarthritis
Tissue
Feedback
Messenger RNA

Keywords

  • CILP
  • Responsive element
  • Smad3
  • TGF-β1
  • Transcription

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene. / Mori, Masaki; Nakajima, Masahiro; Mikami, Yasuo; Seki, Shoji; Takigawa, Masaharu; Kubo, Toshikazu; Ikegawa, Shiro.

In: Biochemical and Biophysical Research Communications, Vol. 341, No. 1, 03.03.2006, p. 121-127.

Research output: Contribution to journalArticle

Mori, Masaki ; Nakajima, Masahiro ; Mikami, Yasuo ; Seki, Shoji ; Takigawa, Masaharu ; Kubo, Toshikazu ; Ikegawa, Shiro. / Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene. In: Biochemical and Biophysical Research Communications. 2006 ; Vol. 341, No. 1. pp. 121-127.
@article{423acd020d57451faf724125559ca9dd,
title = "Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene",
abstract = "Cartilage intermediate layer protein (CILP) is an extracellular matrix protein abundant in cartilaginous tissues. CILP is implicated in common musculoskeletal disorders, including osteoarthritis and lumbar disc disease. Regulation of the CILP gene is largely unknown, however. We have found that CILP mRNA expression is induced by TGF-β1 and dependent upon signaling via TGF-β receptors. TGF-β1 induction of CILP is mediated by Smad3, which acts directly through cis-elements in the CILP promoter region. Pathways other than Smad3 also are involved in TGF-β1 induction of CILP. These observations, together with the finding that CILP protein binds and inhibits TGF-β1, suggest that CILP and TGF-β1 may form a functional feedback loop that controls chondrocyte metabolism.",
keywords = "CILP, Responsive element, Smad3, TGF-β1, Transcription",
author = "Masaki Mori and Masahiro Nakajima and Yasuo Mikami and Shoji Seki and Masaharu Takigawa and Toshikazu Kubo and Shiro Ikegawa",
year = "2006",
month = "3",
day = "3",
doi = "10.1016/j.bbrc.2005.12.159",
language = "English",
volume = "341",
pages = "121--127",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Academic Press Inc.",
number = "1",

}

TY - JOUR

T1 - Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene

AU - Mori, Masaki

AU - Nakajima, Masahiro

AU - Mikami, Yasuo

AU - Seki, Shoji

AU - Takigawa, Masaharu

AU - Kubo, Toshikazu

AU - Ikegawa, Shiro

PY - 2006/3/3

Y1 - 2006/3/3

N2 - Cartilage intermediate layer protein (CILP) is an extracellular matrix protein abundant in cartilaginous tissues. CILP is implicated in common musculoskeletal disorders, including osteoarthritis and lumbar disc disease. Regulation of the CILP gene is largely unknown, however. We have found that CILP mRNA expression is induced by TGF-β1 and dependent upon signaling via TGF-β receptors. TGF-β1 induction of CILP is mediated by Smad3, which acts directly through cis-elements in the CILP promoter region. Pathways other than Smad3 also are involved in TGF-β1 induction of CILP. These observations, together with the finding that CILP protein binds and inhibits TGF-β1, suggest that CILP and TGF-β1 may form a functional feedback loop that controls chondrocyte metabolism.

AB - Cartilage intermediate layer protein (CILP) is an extracellular matrix protein abundant in cartilaginous tissues. CILP is implicated in common musculoskeletal disorders, including osteoarthritis and lumbar disc disease. Regulation of the CILP gene is largely unknown, however. We have found that CILP mRNA expression is induced by TGF-β1 and dependent upon signaling via TGF-β receptors. TGF-β1 induction of CILP is mediated by Smad3, which acts directly through cis-elements in the CILP promoter region. Pathways other than Smad3 also are involved in TGF-β1 induction of CILP. These observations, together with the finding that CILP protein binds and inhibits TGF-β1, suggest that CILP and TGF-β1 may form a functional feedback loop that controls chondrocyte metabolism.

KW - CILP

KW - Responsive element

KW - Smad3

KW - TGF-β1

KW - Transcription

UR - http://www.scopus.com/inward/record.url?scp=30944450773&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=30944450773&partnerID=8YFLogxK

U2 - 10.1016/j.bbrc.2005.12.159

DO - 10.1016/j.bbrc.2005.12.159

M3 - Article

VL - 341

SP - 121

EP - 127

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

IS - 1

ER -