Transcriptional regulation of the cartilage intermediate layer protein (CILP) gene

Masaki Mori, Masahiro Nakajima, Yasuo Mikami, Shoji Seki, Masaharu Takigawa, Toshikazu Kubo, Shiro Ikegawa

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Cartilage intermediate layer protein (CILP) is an extracellular matrix protein abundant in cartilaginous tissues. CILP is implicated in common musculoskeletal disorders, including osteoarthritis and lumbar disc disease. Regulation of the CILP gene is largely unknown, however. We have found that CILP mRNA expression is induced by TGF-β1 and dependent upon signaling via TGF-β receptors. TGF-β1 induction of CILP is mediated by Smad3, which acts directly through cis-elements in the CILP promoter region. Pathways other than Smad3 also are involved in TGF-β1 induction of CILP. These observations, together with the finding that CILP protein binds and inhibits TGF-β1, suggest that CILP and TGF-β1 may form a functional feedback loop that controls chondrocyte metabolism.

Original languageEnglish
Pages (from-to)121-127
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume341
Issue number1
DOIs
Publication statusPublished - Mar 3 2006

Keywords

  • CILP
  • Responsive element
  • Smad3
  • TGF-β1
  • Transcription

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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