Abstract
We found that transcription of NOR-1 (neuron-derived orphan receptor-1) is induced by the Ca2+/calmodulin-dependent protein kinase IV (CaM-KIV) cascade by differential hybridization screening of a cDNA array using probes prepared from SH-SY5Y cells infected with recombinant adenoviruses expressing Ca2+/CaM-independent mutants of CaM-KIV and CaM-K kinase (CaM-KK). Induction of NOR-1 mRNA expression by the CaM-KIV cascade was confirmed by RT-PCR. Promoter analyses indicate that CaM-KIV cascade response element is located between-162 bp and-42 bp in the 1.7 kb NOR-1 promoter containing triple cAMP response elements. Disruption of each element significantly reduced the promoter activity, indicating the direct transcriptional regulation of NOR-1 by CaM-KIV cascade.
Original language | English |
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Pages (from-to) | 88-92 |
Number of pages | 5 |
Journal | FEBS Letters |
Volume | 522 |
Issue number | 1-3 |
DOIs | |
Publication status | Published - Jul 3 2002 |
Externally published | Yes |
Keywords
- Ca
- Ca/calmodulin-dependent protein kinase IV cascade
- Ca/calmodulin-dependent protein kinase kinase
- Differential screening
- Neuron-derived orphan receptor-1
ASJC Scopus subject areas
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology