Transcriptional regulation of nuclear orphan receptor, NOR-1, by Ca2+/calmodulin-dependent protein kinase cascade

Hiroyuki Inuzuka, Hiroshi Tokumitsu, Naganari Ohkura, Ryoji Kobayashi

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

We found that transcription of NOR-1 (neuron-derived orphan receptor-1) is induced by the Ca2+/calmodulin-dependent protein kinase IV (CaM-KIV) cascade by differential hybridization screening of a cDNA array using probes prepared from SH-SY5Y cells infected with recombinant adenoviruses expressing Ca2+/CaM-independent mutants of CaM-KIV and CaM-K kinase (CaM-KK). Induction of NOR-1 mRNA expression by the CaM-KIV cascade was confirmed by RT-PCR. Promoter analyses indicate that CaM-KIV cascade response element is located between-162 bp and-42 bp in the 1.7 kb NOR-1 promoter containing triple cAMP response elements. Disruption of each element significantly reduced the promoter activity, indicating the direct transcriptional regulation of NOR-1 by CaM-KIV cascade.

Original languageEnglish
Pages (from-to)88-92
Number of pages5
JournalFEBS Letters
Volume522
Issue number1-3
DOIs
Publication statusPublished - Jul 3 2002
Externally publishedYes

Keywords

  • Ca
  • Ca/calmodulin-dependent protein kinase IV cascade
  • Ca/calmodulin-dependent protein kinase kinase
  • Differential screening
  • Neuron-derived orphan receptor-1

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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