Transcriptional regulation of a brown adipocyte-specific gene, UCP1, by KLF11 and KLF15

Ken ichi Yamamoto, Masakiyo Sakaguchi, Reinhold J. Medina, Aya Niida, Yoshihiko Sakaguchi, Masahiro Miyazaki, Ken Kataoka, Nam ho Huh

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

Several growth factors and transcription factors have been reported to play important roles in brown adipocyte differentiation and modulation of thermogenic gene expression, especially the expression of UCP1. In this study, we focused on KLF11 and KLF15, which were expressed highly in brown adipose tissue. Our data demonstrated that KLF11 and KLF15 interacted directly with the UCP1 promoter using GC-box and GT-boxes, respectively. Co-transfection of KLF11 and KLF15 in the mesenchymal stem cell line muBM3.1 during brown adipocyte differentiation enhanced the expression level of UCP1. KLF11, but not KLF15, was essential for UCP1 expression during brown adipocyte differentiation of muBM3.1.

Original languageEnglish
Pages (from-to)175-180
Number of pages6
JournalBiochemical and Biophysical Research Communications
Volume400
Issue number1
DOIs
Publication statusPublished - Sep 2010

Keywords

  • Brown adipocyte
  • KLF11
  • KLF15
  • Promoter
  • UCP1

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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