Transcriptional mapping of the messenger and leader RNAs of orchid fleck virus, a bisegmented negative-strand RNA virus

Hideki Kondo, Kazuyuki Maruyama, Sotaro Chiba, Ida Bagus Andika, Nobuhiro Suzuki

    Research output: Contribution to journalArticlepeer-review

    13 Citations (Scopus)

    Abstract

    The transcriptional strategy of orchid fleck virus (OFV), which has a two-segmented negative-strand RNA genome and resembles plant nucleorhabdoviruses, remains unexplored. In this study, the transcripts of six genes encoded by OFV RNA1 and RNA2 in the poly(A)-enriched RNA fraction from infected plants were molecularly characterized. All of the OFV mRNAs were initiated at a start sequence 3'-UU-5' with one to three non-viral adenine nucleotides which were added at the 5' end of each mRNA, whereas their 3' termini ended with a 5'-AUUUAAA(U/G)AAAA(A). n-3' sequence. We also identified the presence of polyadenylated short transcripts derived from the 3'-terminal leader regions of both genomic and antigenomic strands, providing the first example of plus- and minus-strand leader RNAs in a segmented minus-strand RNA virus. The similarity in the transcriptional strategy between this bipartite OFV and monopartite rhabdoviruses, especially nucleorhabdoviruses (family Rhabdoviridae) is additional support for their close relationship.

    Original languageEnglish
    Pages (from-to)166-174
    Number of pages9
    JournalVirology
    Volume452-453
    DOIs
    Publication statusPublished - Mar 2014

    Keywords

    • Bipartite genome
    • Dichorhavirus
    • Gene junction
    • Leader RNA
    • Negative-strand RNA virus
    • Orchid fleck virus
    • Polyadenylated
    • RACE
    • Rhabdovirus
    • Transcription

    ASJC Scopus subject areas

    • Virology

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