Transcriptional control by myb oncogene product.

S. Ishii, T. Nomura, C. Kanei-Ishii, Hideki Nakagoshi, T. Sudo, T. Sawazaki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Structure and function of two domains of c-Myb were analyzed. We show that a leucine zipper structure is a component of the negative regulatory domain, because its disruption markedly increases both the transactivating and transforming capacities of c-Myb. Our results suggest that an inhibitor which suppresses transactivation binds to c-Myb through the leucine zipper, and that c-Myb can be oncogenically activated by mis-sense mutation. We also proposed a model, the "tryptophan cluster", for the structure of the Myb DNA-binding domain, in which the three tryptophans form a cluster in the hydrophobic core in each repeat. The results of NMR analysis of repeat 3 revealed that the conserved tryptophans play a key role to make the hydrophobic core.

Original languageEnglish
Pages (from-to)189-194
Number of pages6
JournalTohoku Journal of Experimental Medicine
Volume168
Issue number2
Publication statusPublished - Oct 1992
Externally publishedYes

Fingerprint

myb Genes
Oncogene Proteins
Tryptophan
Leucine Zippers
Transcriptional Activation
Nuclear magnetic resonance
Mutation
DNA

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Ishii, S., Nomura, T., Kanei-Ishii, C., Nakagoshi, H., Sudo, T., & Sawazaki, T. (1992). Transcriptional control by myb oncogene product. Tohoku Journal of Experimental Medicine, 168(2), 189-194.

Transcriptional control by myb oncogene product. / Ishii, S.; Nomura, T.; Kanei-Ishii, C.; Nakagoshi, Hideki; Sudo, T.; Sawazaki, T.

In: Tohoku Journal of Experimental Medicine, Vol. 168, No. 2, 10.1992, p. 189-194.

Research output: Contribution to journalArticle

Ishii, S, Nomura, T, Kanei-Ishii, C, Nakagoshi, H, Sudo, T & Sawazaki, T 1992, 'Transcriptional control by myb oncogene product.', Tohoku Journal of Experimental Medicine, vol. 168, no. 2, pp. 189-194.
Ishii S, Nomura T, Kanei-Ishii C, Nakagoshi H, Sudo T, Sawazaki T. Transcriptional control by myb oncogene product. Tohoku Journal of Experimental Medicine. 1992 Oct;168(2):189-194.
Ishii, S. ; Nomura, T. ; Kanei-Ishii, C. ; Nakagoshi, Hideki ; Sudo, T. ; Sawazaki, T. / Transcriptional control by myb oncogene product. In: Tohoku Journal of Experimental Medicine. 1992 ; Vol. 168, No. 2. pp. 189-194.
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