Transcriptional analysis of the groESL operon from Porphyromonas gingivalis

H. Hotokezaka, Naoya Oohara, H. Hayashida, S. Matsumoto, T. Matsuo, M. Naito, K. Kobayashi, T. Yamada

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Transcriptional analysis of the groESL operon from Porphyromonas gingivalis, one of the obligative anaerobic oral microorganisms implicated in adult periodontitis, was performed. P. gingivalis 381 cultured at 37°C was shifted to 42°C, 45°C or 48°C for 10 mins. Northern hybridization analysis revealed that a band with 2.1-kb (kilo base pair) was observed, and the transcripts increased greatly by heat shock. Primer extension and S1 mapping detected four different 5'-ending sites of the mRNAs at the upstream region of the groES. Three sites out of the four were heat-inducible. There were inverted repeats and a Escherichia coli σ32-recognizing consensus sequence in the promoter region of the groESL, which may be relevant to the regulation of transcription of groESL operon in P. gingivalis. Both a heat shock promoter and inverted repeats may be relevant to the transcriptional regulation of the groESL operon in P. gingivalis.

Original languageEnglish
Pages (from-to)236-239
Number of pages4
JournalOral Microbiology and Immunology
Volume12
Issue number4
Publication statusPublished - Aug 1997
Externally publishedYes

Fingerprint

Porphyromonas gingivalis
Operon
Hot Temperature
Shock
Chronic Periodontitis
Consensus Sequence
Genetic Promoter Regions
Base Pairing
Escherichia coli
Messenger RNA

Keywords

  • CIRCE
  • GroESL
  • Inverted repeat
  • Northern hybridization
  • Porphyromonas gingivalis
  • Primer extension
  • Promoter
  • S1 mapping
  • Transcription

ASJC Scopus subject areas

  • Immunology
  • Microbiology (medical)
  • Dentistry(all)

Cite this

Hotokezaka, H., Oohara, N., Hayashida, H., Matsumoto, S., Matsuo, T., Naito, M., ... Yamada, T. (1997). Transcriptional analysis of the groESL operon from Porphyromonas gingivalis. Oral Microbiology and Immunology, 12(4), 236-239.

Transcriptional analysis of the groESL operon from Porphyromonas gingivalis. / Hotokezaka, H.; Oohara, Naoya; Hayashida, H.; Matsumoto, S.; Matsuo, T.; Naito, M.; Kobayashi, K.; Yamada, T.

In: Oral Microbiology and Immunology, Vol. 12, No. 4, 08.1997, p. 236-239.

Research output: Contribution to journalArticle

Hotokezaka, H, Oohara, N, Hayashida, H, Matsumoto, S, Matsuo, T, Naito, M, Kobayashi, K & Yamada, T 1997, 'Transcriptional analysis of the groESL operon from Porphyromonas gingivalis', Oral Microbiology and Immunology, vol. 12, no. 4, pp. 236-239.
Hotokezaka H, Oohara N, Hayashida H, Matsumoto S, Matsuo T, Naito M et al. Transcriptional analysis of the groESL operon from Porphyromonas gingivalis. Oral Microbiology and Immunology. 1997 Aug;12(4):236-239.
Hotokezaka, H. ; Oohara, Naoya ; Hayashida, H. ; Matsumoto, S. ; Matsuo, T. ; Naito, M. ; Kobayashi, K. ; Yamada, T. / Transcriptional analysis of the groESL operon from Porphyromonas gingivalis. In: Oral Microbiology and Immunology. 1997 ; Vol. 12, No. 4. pp. 236-239.
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