Abstract
Transcriptional analysis of the groESL operon from Porphyromonas gingivalis, one of the obligative anaerobic oral microorganisms implicated in adult periodontitis, was performed. P. gingivalis 381 cultured at 37°C was shifted to 42°C, 45°C or 48°C for 10 mins. Northern hybridization analysis revealed that a band with 2.1-kb (kilo base pair) was observed, and the transcripts increased greatly by heat shock. Primer extension and S1 mapping detected four different 5'-ending sites of the mRNAs at the upstream region of the groES. Three sites out of the four were heat-inducible. There were inverted repeats and a Escherichia coli σ32-recognizing consensus sequence in the promoter region of the groESL, which may be relevant to the regulation of transcription of groESL operon in P. gingivalis. Both a heat shock promoter and inverted repeats may be relevant to the transcriptional regulation of the groESL operon in P. gingivalis.
| Original language | English |
|---|---|
| Pages (from-to) | 236-239 |
| Number of pages | 4 |
| Journal | Oral Microbiology and Immunology |
| Volume | 12 |
| Issue number | 4 |
| Publication status | Published - Aug 1997 |
| Externally published | Yes |
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Keywords
- CIRCE
- GroESL
- Inverted repeat
- Northern hybridization
- Porphyromonas gingivalis
- Primer extension
- Promoter
- S1 mapping
- Transcription
ASJC Scopus subject areas
- Immunology
- Microbiology (medical)
- Dentistry(all)
Cite this
Transcriptional analysis of the groESL operon from Porphyromonas gingivalis. / Hotokezaka, H.; Oohara, Naoya; Hayashida, H.; Matsumoto, S.; Matsuo, T.; Naito, M.; Kobayashi, K.; Yamada, T.
In: Oral Microbiology and Immunology, Vol. 12, No. 4, 08.1997, p. 236-239.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Transcriptional analysis of the groESL operon from Porphyromonas gingivalis
AU - Hotokezaka, H.
AU - Oohara, Naoya
AU - Hayashida, H.
AU - Matsumoto, S.
AU - Matsuo, T.
AU - Naito, M.
AU - Kobayashi, K.
AU - Yamada, T.
PY - 1997/8
Y1 - 1997/8
N2 - Transcriptional analysis of the groESL operon from Porphyromonas gingivalis, one of the obligative anaerobic oral microorganisms implicated in adult periodontitis, was performed. P. gingivalis 381 cultured at 37°C was shifted to 42°C, 45°C or 48°C for 10 mins. Northern hybridization analysis revealed that a band with 2.1-kb (kilo base pair) was observed, and the transcripts increased greatly by heat shock. Primer extension and S1 mapping detected four different 5'-ending sites of the mRNAs at the upstream region of the groES. Three sites out of the four were heat-inducible. There were inverted repeats and a Escherichia coli σ32-recognizing consensus sequence in the promoter region of the groESL, which may be relevant to the regulation of transcription of groESL operon in P. gingivalis. Both a heat shock promoter and inverted repeats may be relevant to the transcriptional regulation of the groESL operon in P. gingivalis.
AB - Transcriptional analysis of the groESL operon from Porphyromonas gingivalis, one of the obligative anaerobic oral microorganisms implicated in adult periodontitis, was performed. P. gingivalis 381 cultured at 37°C was shifted to 42°C, 45°C or 48°C for 10 mins. Northern hybridization analysis revealed that a band with 2.1-kb (kilo base pair) was observed, and the transcripts increased greatly by heat shock. Primer extension and S1 mapping detected four different 5'-ending sites of the mRNAs at the upstream region of the groES. Three sites out of the four were heat-inducible. There were inverted repeats and a Escherichia coli σ32-recognizing consensus sequence in the promoter region of the groESL, which may be relevant to the regulation of transcription of groESL operon in P. gingivalis. Both a heat shock promoter and inverted repeats may be relevant to the transcriptional regulation of the groESL operon in P. gingivalis.
KW - CIRCE
KW - GroESL
KW - Inverted repeat
KW - Northern hybridization
KW - Porphyromonas gingivalis
KW - Primer extension
KW - Promoter
KW - S1 mapping
KW - Transcription
UR - http://www.scopus.com/inward/record.url?scp=0031214680&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0031214680&partnerID=8YFLogxK
M3 - Article
C2 - 9467393
AN - SCOPUS:0031214680
VL - 12
SP - 236
EP - 239
JO - Molecular Oral Microbiology
JF - Molecular Oral Microbiology
SN - 2041-1006
IS - 4
ER -