Abstract
Transcriptional analysis of the groESL operon from Porphyromonas gingivalis, one of the obligative anaerobic oral microorganisms implicated in adult periodontitis, was performed. P. gingivalis 381 cultured at 37°C was shifted to 42°C, 45°C or 48°C for 10 mins. Northern hybridization analysis revealed that a band with 2.1-kb (kilo base pair) was observed, and the transcripts increased greatly by heat shock. Primer extension and S1 mapping detected four different 5'-ending sites of the mRNAs at the upstream region of the groES. Three sites out of the four were heat-inducible. There were inverted repeats and a Escherichia coli σ32-recognizing consensus sequence in the promoter region of the groESL, which may be relevant to the regulation of transcription of groESL operon in P. gingivalis. Both a heat shock promoter and inverted repeats may be relevant to the transcriptional regulation of the groESL operon in P. gingivalis.
| Original language | English |
|---|---|
| Pages (from-to) | 236-239 |
| Number of pages | 4 |
| Journal | Oral microbiology and immunology |
| Volume | 12 |
| Issue number | 4 |
| DOIs | |
| Publication status | Published - Aug 1997 |
| Externally published | Yes |
Keywords
- CIRCE
- GroESL
- Inverted repeat
- Northern hybridization
- Porphyromonas gingivalis
- Primer extension
- Promoter
- S1 mapping
- Transcription
ASJC Scopus subject areas
- Microbiology
- Immunology
- Dentistry(all)
- Microbiology (medical)