Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes

Yujin Inayoshi, Yuuki Okino, Katsuhide Miyake, Akifumi Mizutani, Junko Yamamoto-Kishikawa, Yuya Kinoshita, Yusuke Morimoto, Kazuhito Imamura, Mahboob Morshed, Ken Kono, Toshinari Itoh, Ken Ichi Nishijima, Shinji Iijima

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7 Citations (Scopus)

Abstract

Retroviral integrases associate during the early viral life cycle with preintegration complexes that catalyze the integration of reverse-transcribed viral cDNA into the host chromosomes. Several cellular and viral proteins have been reported to be incorporated in the preintegration complex. This study demonstrates that transcription factor Yin Yang 1 binds to Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases. The results of coimmunoprecipitation and in vitro pulldown assays revealed that Yin Yang 1 interacted with the catalytic core and C-terminal domains of Moloney murine leukemia virus and human immunodeficiency virus type 1 integrases, while the transcriptional repression and DNA-binding domains of the Yin Yang 1 molecule interacted with Moloney murine leukemia virus integrase. Immunoprecipitation of the cytoplasmic fraction of virus-infected cells followed by Southern blotting and chromatin immunoprecipitation demonstrated that Yin Yang 1 associated with Moloney murine leukemia virus cDNA in virus-infected cells. Yin Yang 1 enhanced the in vitro integrase activity of Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases. Furthermore, knockdown of Yin Yang 1 in host cells by small interfering RNA reduced Moloney murine leukemia virus cDNA integration in vivo, although viral cDNA synthesis was increased, suggesting that Yin Yang 1 facilitates integration events in vivo. Taking these results together, Yin Yang 1 appears to be involved in integration events during the early viral life cycle, possibly as an enhancer of integration.

Original languageEnglish
Pages (from-to)8250-8261
Number of pages12
JournalJournal of Virology
Volume84
Issue number16
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

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ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Inayoshi, Y., Okino, Y., Miyake, K., Mizutani, A., Yamamoto-Kishikawa, J., Kinoshita, Y., Morimoto, Y., Imamura, K., Morshed, M., Kono, K., Itoh, T., Nishijima, K. I., & Iijima, S. (2010). Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes. Journal of Virology, 84(16), 8250-8261. https://doi.org/10.1128/JVI.02681-09