Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes

Yujin Inayoshi, Yuuki Okino, Katsuhide Miyake, Akifumi Mizutani, Junko Yamamoto-Kishikawa, Yuya Kinoshita, Yusuke Morimoto, Kazuhito Imamura, Mahboob Morshed, Ken Kono, Toshinari Itoh, Ken Ichi Nishijima, Shinji Iijima

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Retroviral integrases associate during the early viral life cycle with preintegration complexes that catalyze the integration of reverse-transcribed viral cDNA into the host chromosomes. Several cellular and viral proteins have been reported to be incorporated in the preintegration complex. This study demonstrates that transcription factor Yin Yang 1 binds to Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases. The results of coimmunoprecipitation and in vitro pulldown assays revealed that Yin Yang 1 interacted with the catalytic core and C-terminal domains of Moloney murine leukemia virus and human immunodeficiency virus type 1 integrases, while the transcriptional repression and DNA-binding domains of the Yin Yang 1 molecule interacted with Moloney murine leukemia virus integrase. Immunoprecipitation of the cytoplasmic fraction of virus-infected cells followed by Southern blotting and chromatin immunoprecipitation demonstrated that Yin Yang 1 associated with Moloney murine leukemia virus cDNA in virus-infected cells. Yin Yang 1 enhanced the in vitro integrase activity of Moloney murine leukemia virus, human immunodeficiency virus type 1, and avian sarcoma virus integrases. Furthermore, knockdown of Yin Yang 1 in host cells by small interfering RNA reduced Moloney murine leukemia virus cDNA integration in vivo, although viral cDNA synthesis was increased, suggesting that Yin Yang 1 facilitates integration events in vivo. Taking these results together, Yin Yang 1 appears to be involved in integration events during the early viral life cycle, possibly as an enhancer of integration.

Original languageEnglish
Pages (from-to)8250-8261
Number of pages12
JournalJournal of Virology
Volume84
Issue number16
DOIs
Publication statusPublished - Aug 2010
Externally publishedYes

Fingerprint

YY1 Transcription Factor
Murine leukemia virus
Yin-Yang
Moloney murine leukemia virus
Integrases
transcription factors
Complementary DNA
Chromosomes
chromosomes
Human immunodeficiency virus 1
Rous sarcoma virus
Avian Sarcoma Viruses
HIV-1
life cycle (organisms)
Life Cycle Stages
viruses
DNA-binding domains
viral proteins
cells
Virus Integration

ASJC Scopus subject areas

  • Immunology
  • Virology

Cite this

Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes. / Inayoshi, Yujin; Okino, Yuuki; Miyake, Katsuhide; Mizutani, Akifumi; Yamamoto-Kishikawa, Junko; Kinoshita, Yuya; Morimoto, Yusuke; Imamura, Kazuhito; Morshed, Mahboob; Kono, Ken; Itoh, Toshinari; Nishijima, Ken Ichi; Iijima, Shinji.

In: Journal of Virology, Vol. 84, No. 16, 08.2010, p. 8250-8261.

Research output: Contribution to journalArticle

Inayoshi, Y, Okino, Y, Miyake, K, Mizutani, A, Yamamoto-Kishikawa, J, Kinoshita, Y, Morimoto, Y, Imamura, K, Morshed, M, Kono, K, Itoh, T, Nishijima, KI & Iijima, S 2010, 'Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes', Journal of Virology, vol. 84, no. 16, pp. 8250-8261. https://doi.org/10.1128/JVI.02681-09
Inayoshi, Yujin ; Okino, Yuuki ; Miyake, Katsuhide ; Mizutani, Akifumi ; Yamamoto-Kishikawa, Junko ; Kinoshita, Yuya ; Morimoto, Yusuke ; Imamura, Kazuhito ; Morshed, Mahboob ; Kono, Ken ; Itoh, Toshinari ; Nishijima, Ken Ichi ; Iijima, Shinji. / Transcription factor YY1 interacts with retroviral integrases and facilitates integration of moloney murine leukemia virus cDNA into the host chromosomes. In: Journal of Virology. 2010 ; Vol. 84, No. 16. pp. 8250-8261.
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