Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae

Yoshimi Matsufuji, Tomoyuki Nakagawa, Shuki Fujimura, Akio Tani, Junichi Nakagawa

    Research output: Contribution to journalArticlepeer-review

    8 Citations (Scopus)

    Abstract

    Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.

    Original languageEnglish
    Pages (from-to)494-498
    Number of pages5
    JournalJournal of basic microbiology
    Volume50
    Issue number5
    DOIs
    Publication statusPublished - Oct 1 2010

    Keywords

    • Glycolysis
    • Pentose phosphate pathway
    • STB5, Acetaldehyde tolerance
    • Saccharomyces cerevisiae

    ASJC Scopus subject areas

    • Applied Microbiology and Biotechnology

    Fingerprint

    Dive into the research topics of 'Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae'. Together they form a unique fingerprint.

    Cite this