Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae

Yoshimi Matsufuji, Tomoyuki Nakagawa, Shuki Fujimura, Akiwo Tani, Junichi Nakagawa

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.

Original languageEnglish
Pages (from-to)494-498
Number of pages5
JournalJournal of Basic Microbiology
Volume50
Issue number5
DOIs
Publication statusPublished - Oct 2010

Fingerprint

Acetaldehyde
Saccharomyces cerevisiae
Transcription Factors
Pentose Phosphate Pathway
Glucose-6-Phosphate Isomerase
MDR Genes
Gene Deletion
Glycolysis
Genes
Down-Regulation
Yeasts

Keywords

  • Glycolysis
  • Pentose phosphate pathway
  • Saccharomyces cerevisiae
  • STB5, Acetaldehyde tolerance

ASJC Scopus subject areas

  • Applied Microbiology and Biotechnology

Cite this

Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae. / Matsufuji, Yoshimi; Nakagawa, Tomoyuki; Fujimura, Shuki; Tani, Akiwo; Nakagawa, Junichi.

In: Journal of Basic Microbiology, Vol. 50, No. 5, 10.2010, p. 494-498.

Research output: Contribution to journalArticle

Matsufuji, Yoshimi ; Nakagawa, Tomoyuki ; Fujimura, Shuki ; Tani, Akiwo ; Nakagawa, Junichi. / Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae. In: Journal of Basic Microbiology. 2010 ; Vol. 50, No. 5. pp. 494-498.
@article{a01d659ea6da42139c599a84b73db6b1,
title = "Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae",
abstract = "Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.",
keywords = "Glycolysis, Pentose phosphate pathway, Saccharomyces cerevisiae, STB5, Acetaldehyde tolerance",
author = "Yoshimi Matsufuji and Tomoyuki Nakagawa and Shuki Fujimura and Akiwo Tani and Junichi Nakagawa",
year = "2010",
month = "10",
doi = "10.1002/jobm.200900391",
language = "English",
volume = "50",
pages = "494--498",
journal = "Journal of Basic Microbiology",
issn = "0233-111X",
publisher = "Wiley-VCH Verlag",
number = "5",

}

TY - JOUR

T1 - Transcription factor Stb5p is essential for acetaldehyde tolerance in Saccharomyces cerevisiae

AU - Matsufuji, Yoshimi

AU - Nakagawa, Tomoyuki

AU - Fujimura, Shuki

AU - Tani, Akiwo

AU - Nakagawa, Junichi

PY - 2010/10

Y1 - 2010/10

N2 - Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.

AB - Transcription factor Stb5p, previously known as one of the multidrug resistance gene regulators in Saccharomyces cerevisiae, was shown here to play an essential role in acetaldehyde tolerance. A mutant strain, δstb5 exhibited increased acetaldehyde sensitivity, and failed to induce genes such as GND1, TKL1 and TAL1 involved in the pentose phosphate pathway (PPP) upon acetaldehyde stress. Using this strain it was revealed that Stb5p acts as a repressor for PGI1 encoding glucose-6-phosphate isomerase under acetaldehyde stress. In reverse, over-expression of Stb5p reinforced acetaldehyde tolerance to the yeast. Furthermore, various deletion mutants of the genes involved in glycolysis showed increased acetaldehyde tolerance compared to the wild-type strain. From these results, it was suggested that Stb5p participates in acetaldehyde tolerance by regulating expression of the PPP genes and PGI1, and that down-regulation of glycolytic pathway may lead to vitalization of PPP and to increased acetaldehyde tolerance.

KW - Glycolysis

KW - Pentose phosphate pathway

KW - Saccharomyces cerevisiae

KW - STB5, Acetaldehyde tolerance

UR - http://www.scopus.com/inward/record.url?scp=77958587281&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=77958587281&partnerID=8YFLogxK

U2 - 10.1002/jobm.200900391

DO - 10.1002/jobm.200900391

M3 - Article

C2 - 20806246

AN - SCOPUS:77958587281

VL - 50

SP - 494

EP - 498

JO - Journal of Basic Microbiology

JF - Journal of Basic Microbiology

SN - 0233-111X

IS - 5

ER -