Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence

Chikara Kaito, Yuki Saito, Gentaro Nagano, Mariko Ikuo, Yosuke Omae, Yuichi Hanada, Xiao Han, Kyoko Kuwahara-Arai, Tomomi Hishinuma, Tadashi Baba, Teruyo Ito, Keiichi Hiramatsu, Kazuhisa Sekimizu

Research output: Contribution to journalArticle

74 Citations (Scopus)

Abstract

The F region downstream of the mecI gene in the SCCmec element in hospital-associated methicillin-resistant Staphylococcus aureus (HA-MRSA) contains two bidirectionally overlapping open reading frames (ORFs), the fudoh ORF and the psm-mec ORF. The psm-mec ORF encodes a cytolysin, phenol-soluble modulin (PSM)-mec. Transformation of the F region into the Newman strain, which is a methicillin-sensitive S. aureus (MSSA) strain, or into the MW2 (USA400) and FRP3757 (USA300) strains, which are community-acquired MRSA (CA-MRSA) strains that lack the F region, attenuated their virulence in a mouse systemic infection model. Introducing the F region to these strains suppressed colony-spreading activity and PSMα production, and promoted biofilm formation. By producing mutations into the psm-mec ORF, we revealed that (i) both the transcription and translation products of the psm-mec ORF suppressed colony-spreading activity and promoted biofilm formation; and (ii) the transcription product of the psm-mec ORF, but not its translation product, decreased PSMα production. These findings suggest that both the psm-mec transcript, acting as a regulatory RNA, and the PSM-mec protein encoded by the gene on the mobile genetic element SCCmec regulate the virulence of Staphylococcus aureus.

Original languageEnglish
Article numbere1001267
JournalPLoS Pathogens
Volume7
Issue number2
DOIs
Publication statusPublished - Feb 1 2011
Externally publishedYes

Fingerprint

Interspersed Repetitive Sequences
Perforin
Open Reading Frames
Virulence
Staphylococcus aureus
Genes
Methicillin-Resistant Staphylococcus aureus
Biofilms
Methicillin
RNA
Mutation
staphylococcal delta toxin
Infection

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Molecular Biology
  • Genetics
  • Virology

Cite this

Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. / Kaito, Chikara; Saito, Yuki; Nagano, Gentaro; Ikuo, Mariko; Omae, Yosuke; Hanada, Yuichi; Han, Xiao; Kuwahara-Arai, Kyoko; Hishinuma, Tomomi; Baba, Tadashi; Ito, Teruyo; Hiramatsu, Keiichi; Sekimizu, Kazuhisa.

In: PLoS Pathogens, Vol. 7, No. 2, e1001267, 01.02.2011.

Research output: Contribution to journalArticle

Kaito, C, Saito, Y, Nagano, G, Ikuo, M, Omae, Y, Hanada, Y, Han, X, Kuwahara-Arai, K, Hishinuma, T, Baba, T, Ito, T, Hiramatsu, K & Sekimizu, K 2011, 'Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence', PLoS Pathogens, vol. 7, no. 2, e1001267. https://doi.org/10.1371/journal.ppat.1001267
Kaito, Chikara ; Saito, Yuki ; Nagano, Gentaro ; Ikuo, Mariko ; Omae, Yosuke ; Hanada, Yuichi ; Han, Xiao ; Kuwahara-Arai, Kyoko ; Hishinuma, Tomomi ; Baba, Tadashi ; Ito, Teruyo ; Hiramatsu, Keiichi ; Sekimizu, Kazuhisa. / Transcription and translation products of the cytolysin gene psm-mec on the mobile genetic element SCCmec regulate Staphylococcus aureus virulence. In: PLoS Pathogens. 2011 ; Vol. 7, No. 2.
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