Background: Although the cellular and molecular biological effects of interferon (IFN)-α have been well-investigated, the effects of IFN-γ are less understood. Materials and Methods: Eleven human myeloma cell lines with various myeloma-specific chromosomal translocations and overexpression of oncogenes were cultured with 1,000 U/ml of IFN-γ. In the KMS-20 cells, which showed growth inhibition due to IFN-γ, trail expression, status of the Janus kinase (JAK)/STAT pathway were analyzed. Results: KMS-20 cells showed marked up-regulation of trail, activation of STAT1 and TRAIL hyperproduction induced by IFN-γ. Conclusion: The effects of IFN-γ on growth inhibition of KMS-20 cells were characterized by activation of the JAK/STAT signalling pathway, particularly STAT1 phosphorylation, enhanced secretion of TRAIL, and auto/paracrine usage of secreted TRAIL to induce apoptotic cell death. From these results, IFN-γ may be considered one of the drugs to be used in future multidrug chemotherapeutic regimens for myeloma patients.
|Number of pages||10|
|Issue number||6 B|
|Publication status||Published - Nov 1 2006|
ASJC Scopus subject areas
- Cancer Research