Traffic jam on the cellular secretory pathway generated by a replication protein from a plant RNA virus

Kiwamu Hyoudou, Masanori Kaido, Tetsuro Okuno

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Although positive-strand RNA [(+)RNA] viruses have a limited coding capacity, they can replicate efficiently in host cells because of their ability to use host-derived proteins, membranes, lipids, and metabolites, and to rewire cellular trafficking pathways. Previously, we showed that a plant RNA virus, the Red clover necrotic mosaic virus (RCNMV), hijacked Arf1 and Sar1, which are small GTPases that regulate the biogenesis of COPI and COPII vesicles, respectively, for viral RNA replication. These small GTPases are relocated from appropriate subcellular compartments to the viral RNA replication sites by p27 replication protein, which raises the possibility that RCNMV interferes with the cellular secretory pathway. Here, we examined this possibility by using green fluorescent protein-fused rice SCAMP1 and Arabidopsis LRR84A as secretory pathway marker proteins and showed that p27 inhibited the trafficking of these proteins. RCNMV-mediated inhibition of the host secretion pathway and its possible impact on plant-virus interaction are discussed.

Original languageEnglish
Pages (from-to)e28644
JournalPlant Signaling and Behavior
Volume9
Issue number3
DOIs
Publication statusPublished - 2014
Externally publishedYes

Fingerprint

Red clover necrotic mosaic virus
jams
traffic
guanosinetriphosphatase
protein transport
proteins
plant viruses
green fluorescent protein
membrane proteins
Arabidopsis
secretion
RNA
metabolites
rice
lipids
RNA viruses
cells
RNA replication

Keywords

  • Arf1
  • COPI
  • COPII
  • ER
  • golgi
  • intracellular membrane
  • membrane trafficking
  • plant RNA virus
  • replication protein
  • Sar1

ASJC Scopus subject areas

  • Plant Science

Cite this

Traffic jam on the cellular secretory pathway generated by a replication protein from a plant RNA virus. / Hyoudou, Kiwamu; Kaido, Masanori; Okuno, Tetsuro.

In: Plant Signaling and Behavior, Vol. 9, No. 3, 2014, p. e28644.

Research output: Contribution to journalArticle

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