Tracing of the molecular remnants of herpes virus infections in necrotic skin tissue

Takenobu Yamamoto, Akiko Yamada, Kazuhide Tsuji, Keiji Iwatsuki

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

We have established an assay system to detect herpesvirus-derived transcripts in lesional crusts. Fifteen patients with herpes simplex (HS), 21 with herpes zoster (HZ), 2 with varicella, and 20 with irrelevant diseases were enrolled in the present study. Total RNA was extracted from crusts or scales, and converted to cDNA. Virus-encoded transcripts were amplified using reverse transcriptase (RT)-PCR. Housekeeping gene transcripts such as beta2-microglobulin (β2-MG) and beta-actin (β-actin) mRNA were also examined, and an efficient preservative condition of the crusts was determined. With extracted RNAs, β2-MG and β-actin mRNA were successfully amplified in all crust samples. Herpes simplex virus (HSV)-specific, lytic cycle-related transcript, UL30 mRNA was detected in all 15 HS samples, including 13 samples of HSV-1- and 2 of HSV-2-encoded UL30 mRNA, respectively. Of 23 samples, including 21 HZ and 2 varicella cases, varicella zoster virus (VZV)-specific, lytic cycle-related transcript, ORF40 mRNA was detected in 22 samples. In a control group, no UL30 and ORF40 mRNA were detected. Crust samples that had been stored without any pretreatment or preservative for 6 months at room temperature (RT) were available for the present assay. When compared with the freshly obtained materials, the amount of β2-MG mRNA was reduced to 51% in the stored samples covered with adhesive tape, to 48% in a sample left at R.T. without any treatment, and to 1.2% in the samples stocked in saline for 5 days. Herpes virus- and host-derived transcripts contained in crusts can be detected by RT-PCR amplification. Crusts or dry epidermal necrosis with inflammatory cells may provide beneficial diagnostic information.

Original languageEnglish
Pages (from-to)499-503
Number of pages5
JournalEuropean Journal of Dermatology
Volume18
Issue number5
DOIs
Publication statusPublished - Sep 2008

Fingerprint

Virus Diseases
Messenger RNA
Skin
Herpes Simplex
Human Herpesvirus 2
Chickenpox
Herpes Zoster
Reverse Transcriptase Polymerase Chain Reaction
Actins
RNA
Viruses
Human Herpesvirus 3
Herpesviridae
Essential Genes
Human Herpesvirus 1
Simplexvirus
Adhesives
Necrosis
Complementary DNA
Control Groups

Keywords

  • Herpes simplex
  • Herpes simplex virus
  • Herpes zoster
  • Lytic infection
  • RNA
  • Varicella-zoster virus

ASJC Scopus subject areas

  • Dermatology

Cite this

Tracing of the molecular remnants of herpes virus infections in necrotic skin tissue. / Yamamoto, Takenobu; Yamada, Akiko; Tsuji, Kazuhide; Iwatsuki, Keiji.

In: European Journal of Dermatology, Vol. 18, No. 5, 09.2008, p. 499-503.

Research output: Contribution to journalArticle

Yamamoto, Takenobu ; Yamada, Akiko ; Tsuji, Kazuhide ; Iwatsuki, Keiji. / Tracing of the molecular remnants of herpes virus infections in necrotic skin tissue. In: European Journal of Dermatology. 2008 ; Vol. 18, No. 5. pp. 499-503.
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