TPR domain of Ser/Thr phosphatase of Aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity

Bin Feng, Chun Hui Zhao, Soukichi Tanaka, Hiroyuki Imanaka, Koreyoshi Imamura, Kazuhiro Nakanishi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A Ser/Thr phosphatase gene cloned from Aspergillus oryzae, aoppt, revealed that the tetratricopeptide repeat (TPR) and catalytic domains of the full-length AoPPT are located at the N- and C-terminal regions, respectively, similar to those of human Ser/Thr phosphatase 5 (PP5) and yeast Ppt1. Four different regions of AoPPT, namely, a full-length polypeptide, the catalytic domain, the catalytic domain plus C-terminal 15 amino-acid residues and the TPR domain were expressed in Escherichia coli and their roles in dephosphorylation activity were examined, using p-nitrophenyl phosphate as the substrate. The full-length AoPPT showed the highest dephosphorylation activity while the catalytic domain had the lowest activity. The activity of the catalytic domain was not inhibited by the presence of the TPR domain and arachidonic acid did not increase the activity of the full-length enzyme. These findings suggest that the integrity of the entire enzyme would be necessary for its full activity to be expressed.

Original languageEnglish
Pages (from-to)281-285
Number of pages5
JournalInternational Journal of Biological Macromolecules
Volume41
Issue number3
DOIs
Publication statusPublished - Aug 1 2007

Fingerprint

Aspergillus oryzae
Aspergillus
Phosphoric Monoester Hydrolases
Catalytic Domain
Enzymes
Arachidonic Acid
Yeast
Escherichia coli
Genes
Amino Acids
Peptides
Substrates
Yeasts
nitrophenylphosphate

Keywords

  • AoPPT
  • Aspergillus oryzae
  • Ser/Thr phosphatase
  • TPR domain

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

TPR domain of Ser/Thr phosphatase of Aspergillus oryzae shows no auto-inhibitory effect on the dephosphorylation activity. / Feng, Bin; Zhao, Chun Hui; Tanaka, Soukichi; Imanaka, Hiroyuki; Imamura, Koreyoshi; Nakanishi, Kazuhiro.

In: International Journal of Biological Macromolecules, Vol. 41, No. 3, 01.08.2007, p. 281-285.

Research output: Contribution to journalArticle

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AU - Zhao, Chun Hui

AU - Tanaka, Soukichi

AU - Imanaka, Hiroyuki

AU - Imamura, Koreyoshi

AU - Nakanishi, Kazuhiro

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