Abstract
Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria. Copyright
| Original language | English |
|---|---|
| Article number | 2 |
| Journal | Acta Medica Okayama |
| Volume | 62 |
| Issue number | 6 |
| Publication status | Published - Dec 2008 |
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Keywords
- Aeromonas
- Salinity
- Toxin
- Water
ASJC Scopus subject areas
- Medicine(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
Toxin production by Aeromonas sobria in natural environments : River water vs. seawater. / Khan, Rasel; Takahashi, Eizo; Nakura, Hironori; Ansaruzzaman, Mohammad; Banik, Sukalyani; Ramamurthy, Thandavarayan; Okamoto, Keinosuke.
In: Acta Medica Okayama, Vol. 62, No. 6, 2, 12.2008.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Toxin production by Aeromonas sobria in natural environments
T2 - River water vs. seawater
AU - Khan, Rasel
AU - Takahashi, Eizo
AU - Nakura, Hironori
AU - Ansaruzzaman, Mohammad
AU - Banik, Sukalyani
AU - Ramamurthy, Thandavarayan
AU - Okamoto, Keinosuke
PY - 2008/12
Y1 - 2008/12
N2 - Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria. Copyright
AB - Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria. Copyright
KW - Aeromonas
KW - Salinity
KW - Toxin
KW - Water
UR - http://www.scopus.com/inward/record.url?scp=58149462487&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=58149462487&partnerID=8YFLogxK
M3 - Article
C2 - 19122681
AN - SCOPUS:58149462487
VL - 62
JO - Acta Medica Okayama
JF - Acta Medica Okayama
SN - 0386-300X
IS - 6
M1 - 2
ER -