Toxin production by Aeromonas sobria in natural environments: River water vs. seawater

Rasel Khan, Eizo Takahashi, Hironori Nakura, Mohammad Ansaruzzaman, Sukalyani Banik, Thandavarayan Ramamurthy, Keinosuke Okamoto

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Aeromonas are water-borne pathogens. They are halotolerant, which means that they can survive in environments whose salt content corresponds to that of seawater (3.0% NaCl). However, the presence of Aeromonas in seawater is extremely rare compared with that in river water. In this study, we tested the ability of Aeromonas sobria to produce toxins in river water and seawater. First, we cultured A. sobria on skim milk agar plates supplemented with either river water (SARW) or seawater (SASW). The bacteria grew on both plates. A clear zone around the bacteria was generated in SARW. However, such a zone was not observed in SASW, suggesting that proteases were not generated in SASW. Subsequently, we cultured A. sobria in a nutrient broth supplemented with either river water (NRW) or with seawater (NSW), and examined the protease activity of their culture supernatants. The protease activity of the culture supernatant from NSW was extremely low compared to that from NRW. The immunoblotting analysis showed that serine protease (ASP) was not produced by the culture in NSW. By contrast, aerolysin-like hemolysin was produced in all conditions examined in this study. This indicates that the salinity of water is deeply involved in the production of ASP by A. sobria. Copyright

Original languageEnglish
Article number2
JournalActa Medica Okayama
Volume62
Issue number6
Publication statusPublished - Dec 2008

Fingerprint

Aeromonas
Seawater
Rivers
Water
Peptide Hydrolases
Bacteria
Hemolysin Proteins
Salinity
Serine Proteases
Pathogens
Immunoblotting
Nutrients
Agar
Milk
Salts
Food

Keywords

  • Aeromonas
  • Salinity
  • Toxin
  • Water

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Toxin production by Aeromonas sobria in natural environments : River water vs. seawater. / Khan, Rasel; Takahashi, Eizo; Nakura, Hironori; Ansaruzzaman, Mohammad; Banik, Sukalyani; Ramamurthy, Thandavarayan; Okamoto, Keinosuke.

In: Acta Medica Okayama, Vol. 62, No. 6, 2, 12.2008.

Research output: Contribution to journalArticle

Khan, Rasel ; Takahashi, Eizo ; Nakura, Hironori ; Ansaruzzaman, Mohammad ; Banik, Sukalyani ; Ramamurthy, Thandavarayan ; Okamoto, Keinosuke. / Toxin production by Aeromonas sobria in natural environments : River water vs. seawater. In: Acta Medica Okayama. 2008 ; Vol. 62, No. 6.
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