Towards artificial repair of UV-damaged DNA: studies on drug binding and alkali hydrolysis.

Shigenori Iwai, Aki Inase, Sharif Jafar, Miho Higurashi, Takashi Ohtsuki, Yan Xu, Hiroshi Sugiyama

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Properties of the DNA containing the (6-4) photoproduct, one of the major UV-induced lesions, were analyzed. Two basic studies towards artificial recognition and repair of this type of damaged DNA are presented here. One is recognition of the UV-damaged DNA by a minor groove-binding drug. It was found by CD spectroscopy that distamycin could bind DNA duplexes containing the (6-4) photoproduct as effectively as the unmodified DNA, whereas a DNA duplex containing the cyclobutane pyrimidine dimer was not recognized by this drug. The other is a mechanistic study on alkali degradation of this photoproduct. HPLC and NMR analyses revealed that hydrolysis between the N3 and C4 positions of the 5' pyrimidine component occurred first. This intermediate was relatively stable, and further degradation to the strand break required severe conditions like the hot piperidine treatment.

Original languageEnglish
Title of host publicationNucleic acids research. Supplement (2001)
Pages181-182
Number of pages2
Edition3
Publication statusPublished - 2003
Externally publishedYes

Fingerprint

Alkalies
Hydrolysis
DNA
Pharmaceutical Preparations
Pyrimidine Dimers
DNA Repair
Spectrum Analysis
High Pressure Liquid Chromatography

Cite this

Iwai, S., Inase, A., Jafar, S., Higurashi, M., Ohtsuki, T., Xu, Y., & Sugiyama, H. (2003). Towards artificial repair of UV-damaged DNA: studies on drug binding and alkali hydrolysis. In Nucleic acids research. Supplement (2001) (3 ed., pp. 181-182)

Towards artificial repair of UV-damaged DNA : studies on drug binding and alkali hydrolysis. / Iwai, Shigenori; Inase, Aki; Jafar, Sharif; Higurashi, Miho; Ohtsuki, Takashi; Xu, Yan; Sugiyama, Hiroshi.

Nucleic acids research. Supplement (2001). 3. ed. 2003. p. 181-182.

Research output: Chapter in Book/Report/Conference proceedingChapter

Iwai, S, Inase, A, Jafar, S, Higurashi, M, Ohtsuki, T, Xu, Y & Sugiyama, H 2003, Towards artificial repair of UV-damaged DNA: studies on drug binding and alkali hydrolysis. in Nucleic acids research. Supplement (2001). 3 edn, pp. 181-182.
Iwai S, Inase A, Jafar S, Higurashi M, Ohtsuki T, Xu Y et al. Towards artificial repair of UV-damaged DNA: studies on drug binding and alkali hydrolysis. In Nucleic acids research. Supplement (2001). 3 ed. 2003. p. 181-182
Iwai, Shigenori ; Inase, Aki ; Jafar, Sharif ; Higurashi, Miho ; Ohtsuki, Takashi ; Xu, Yan ; Sugiyama, Hiroshi. / Towards artificial repair of UV-damaged DNA : studies on drug binding and alkali hydrolysis. Nucleic acids research. Supplement (2001). 3. ed. 2003. pp. 181-182
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AU - Ohtsuki, Takashi

AU - Xu, Yan

AU - Sugiyama, Hiroshi

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