Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats

Hisataka Miyai, Takayuki Maruyama, Takaaki Tomofuji, Toshiki Yoneda, Tetsuji Azuma, Hirofumi Mizuno, Yoshio Sugiura, Terumasa Kobayashi, Daisuke Ekuni, Manabu Morita

Research output: Contribution to journalArticle

Abstract

Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P < 0.05). Conclusions Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

Original languageEnglish
Pages (from-to)247-255
Number of pages9
JournalArchives of Oral Biology
Volume82
DOIs
Publication statusPublished - Oct 1 2017

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Catechin
Tea
Ointments
Tongue
Fluorouracil
Oxidative Stress
Control Groups
Wistar Rats
Up-Regulation
Fluorescence
Staining and Labeling

Keywords

  • Antioxidant capacity
  • Chemotherapy
  • Green tea catechin
  • Oxidative stress
  • Tongue

ASJC Scopus subject areas

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats. / Miyai, Hisataka; Maruyama, Takayuki; Tomofuji, Takaaki; Yoneda, Toshiki; Azuma, Tetsuji; Mizuno, Hirofumi; Sugiura, Yoshio; Kobayashi, Terumasa; Ekuni, Daisuke; Morita, Manabu.

In: Archives of Oral Biology, Vol. 82, 01.10.2017, p. 247-255.

Research output: Contribution to journalArticle

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abstract = "Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1{\%} or 0.5{\%} green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5{\%} green tea catechin group, but not in the 0.1{\%} green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5{\%} green tea catechin group than in the positive control group (P < 0.05). Conclusions Topical application of ointment containing 0.5{\%} green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.",
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author = "Hisataka Miyai and Takayuki Maruyama and Takaaki Tomofuji and Toshiki Yoneda and Tetsuji Azuma and Hirofumi Mizuno and Yoshio Sugiura and Terumasa Kobayashi and Daisuke Ekuni and Manabu Morita",
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AU - Miyai, Hisataka

AU - Maruyama, Takayuki

AU - Tomofuji, Takaaki

AU - Yoneda, Toshiki

AU - Azuma, Tetsuji

AU - Mizuno, Hirofumi

AU - Sugiura, Yoshio

AU - Kobayashi, Terumasa

AU - Ekuni, Daisuke

AU - Morita, Manabu

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N2 - Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P < 0.05). Conclusions Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

AB - Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P < 0.05). Conclusions Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.

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