TY - JOUR
T1 - Topical application of ointment containing 0.5% green tea catechins suppresses tongue oxidative stress in 5-fluorouracil administered rats
AU - Miyai, Hisataka
AU - Maruyama, Takayuki
AU - Tomofuji, Takaaki
AU - Yoneda, Toshiki
AU - Azuma, Tetsuji
AU - Mizuno, Hirofumi
AU - Sugiura, Yoshio
AU - Kobayashi, Terumasa
AU - Ekuni, Daisuke
AU - Morita, Manabu
N1 - Funding Information:
This work was supported by Grants-in-Aid for Scientific Research (Nos. 26861830, 16K11855 and 16K20693) from the Ministry of Education, Culture, Sports, Science and Technology, Tokyo, Japan.
Publisher Copyright:
© 2017 Elsevier Ltd
PY - 2017/10
Y1 - 2017/10
N2 - Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P < 0.05). Conclusions Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.
AB - Objective The purpose of this study was to investigate the preventive effects of topical application of green tea catechins on tongue oxidative stress induced by 5-fluorouracil (5-FU) administration in rats. Design Male Wistar rats (n = 28, 8 weeks old) were divided into four groups of seven rats each: a negative control group (saline administration and application of ointment without green tea catechins), a positive control group (5-FU administration and application of ointment without green tea catechins), and two experimental groups (5-FU administration and application of ointment containing 0.1% or 0.5% green tea catechins). Topical application of each ointment to the ventral surface of the tongue was performed once a day for 5 days. The level of 8-hydroxydeoxyguanosine (8-OHdG) was determined to evaluate oxidative stress. Fluorescence staining was also performed to confirm nuclear factor erythroid 2-related factor 2 (Nrf2) translocation to the nucleus. Results After the experimental period, the ratios of 8-OHdG-positive cells in the ventral tongue tissue were higher in the positive control group than in the negative control group (P < 0.05). On the other hand, those in the 0.5% green tea catechin group, but not in the 0.1% green tea catechin group, were lower than the positive control group (P < 0.05). In addition, Nrf2 translocation to the nucleus was greater in the 0.5% green tea catechin group than in the positive control group (P < 0.05). Conclusions Topical application of ointment containing 0.5% green tea catechins could prevent tongue oxidative stress in 5-FU administered rats, via up-regulation of the Nrf2 signaling pathway.
KW - Antioxidant capacity
KW - Chemotherapy
KW - Green tea catechin
KW - Oxidative stress
KW - Tongue
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U2 - 10.1016/j.archoralbio.2017.06.025
DO - 10.1016/j.archoralbio.2017.06.025
M3 - Article
C2 - 28668765
AN - SCOPUS:85021442188
VL - 82
SP - 247
EP - 255
JO - Archives of Oral Biology
JF - Archives of Oral Biology
SN - 0003-9969
ER -