Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

W. R. Zhang, H. Kitagawa, T. Hayashi, C. Sasaki, K. Sakai, H. Warita, Y. Shiro, H. Suenaga, H. Ohmae, S. Tsuji, T. Itoh, O. Nishimura, H. Nagasaki, Koji Abe

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalBrain Research
Volume842
Issue number1
DOIs
Publication statusPublished - Sep 18 1999

Fingerprint

Neurotrophin 3
Middle Cerebral Artery Infarction
Brain Injuries
HSP72 Heat-Shock Proteins
DNA Fragmentation
Caspase 3
DNA Nucleotidylexotransferase
In Situ Nick-End Labeling
Biotin
Reperfusion
Cell Count
Staining and Labeling
Wounds and Injuries
Brain

Keywords

  • Caspase-3
  • HSP72
  • Ischemia
  • MCAO
  • Neurotrophin-3
  • TUNEL

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats. / Zhang, W. R.; Kitagawa, H.; Hayashi, T.; Sasaki, C.; Sakai, K.; Warita, H.; Shiro, Y.; Suenaga, H.; Ohmae, H.; Tsuji, S.; Itoh, T.; Nishimura, O.; Nagasaki, H.; Abe, Koji.

In: Brain Research, Vol. 842, No. 1, 18.09.1999, p. 211-214.

Research output: Contribution to journalArticle

Zhang, WR, Kitagawa, H, Hayashi, T, Sasaki, C, Sakai, K, Warita, H, Shiro, Y, Suenaga, H, Ohmae, H, Tsuji, S, Itoh, T, Nishimura, O, Nagasaki, H & Abe, K 1999, 'Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats', Brain Research, vol. 842, no. 1, pp. 211-214. https://doi.org/10.1016/S0006-8993(99)01818-1
Zhang, W. R. ; Kitagawa, H. ; Hayashi, T. ; Sasaki, C. ; Sakai, K. ; Warita, H. ; Shiro, Y. ; Suenaga, H. ; Ohmae, H. ; Tsuji, S. ; Itoh, T. ; Nishimura, O. ; Nagasaki, H. ; Abe, Koji. / Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats. In: Brain Research. 1999 ; Vol. 842, No. 1. pp. 211-214.
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