Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

W. R. Zhang; H. Kitagawa; T. Hayashi; C. Sasaki; K. Sakai; H. Warita; Y. Shiro; H. Suenaga; H. Ohmae; S. Tsuji; T. Itoh; O. Nishimura; H. Nagasaki; K. Abe

doi:10.1016/S0006-8993(99)01818-1

Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

W. R. Zhang, H. Kitagawa, T. Hayashi, C. Sasaki, K. Sakai, H. Warita, Y. Shiro, H. Suenaga, H. Ohmae, S. Tsuji, T. Itoh, O. Nishimura, H. Nagasaki, K. Abe

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Abstract

In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.

Original language	English
Pages (from-to)	211-214
Number of pages	4
Journal	Brain Research
Volume	842
Issue number	1
DOIs	https://doi.org/10.1016/S0006-8993(99)01818-1
Publication status	Published - Sept 18 1999

Keywords

Caspase-3
HSP72
Ischemia
MCAO
Neurotrophin-3
TUNEL

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(99)01818-1

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Zhang, W. R., Kitagawa, H., Hayashi, T., Sasaki, C., Sakai, K., Warita, H., Shiro, Y., Suenaga, H., Ohmae, H., Tsuji, S., Itoh, T., Nishimura, O., Nagasaki, H., & Abe, K. (1999). Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats. Brain Research, 842(1), 211-214. https://doi.org/10.1016/S0006-8993(99)01818-1

Zhang, WR, Kitagawa, H, Hayashi, T, Sasaki, C, Sakai, K, Warita, H, Shiro, Y, Suenaga, H, Ohmae, H, Tsuji, S, Itoh, T, Nishimura, O, Nagasaki, H & Abe, K 1999, 'Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats', Brain Research, vol. 842, no. 1, pp. 211-214. https://doi.org/10.1016/S0006-8993(99)01818-1

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title = "Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats",

abstract = "In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.",

keywords = "Caspase-3, HSP72, Ischemia, MCAO, Neurotrophin-3, TUNEL",

author = "Zhang, {W. R.} and H. Kitagawa and T. Hayashi and C. Sasaki and K. Sakai and H. Warita and Y. Shiro and H. Suenaga and H. Ohmae and S. Tsuji and T. Itoh and O. Nishimura and H. Nagasaki and K. Abe",

note = "Funding Information: This work was partly supported by Grants-in-Aid for Scientific Research nos. 09470151 and 10176204 from the Ministry of Education, Japan, and Takeda Medical Research Foundation.",

year = "1999",

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doi = "10.1016/S0006-8993(99)01818-1",

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AU - Sasaki, C.

AU - Sakai, K.

AU - Warita, H.

AU - Shiro, Y.

AU - Suenaga, H.

AU - Ohmae, H.

AU - Tsuji, S.

AU - Itoh, T.

AU - Nishimura, O.

AU - Nagasaki, H.

AU - Abe, K.

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PY - 1999/9/18

Y1 - 1999/9/18

N2 - In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.

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KW - Caspase-3

KW - HSP72

KW - Ischemia

KW - MCAO

KW - Neurotrophin-3

KW - TUNEL

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Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

Abstract

Keywords

ASJC Scopus subject areas

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