Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats

W. R. Zhang, H. Kitagawa, T. Hayashi, C. Sasaki, K. Sakai, H. Warita, Y. Shiro, H. Suenaga, H. Ohmae, S. Tsuji, T. Itoh, O. Nishimura, H. Nagasaki, K. Abe

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28 Citations (Scopus)

Abstract

In order to examine the effect of neurotrophin-3 (NT-3) on ischemic brain injury, NT-3 was topically applied to brain surface just after 90 min of middle cerebral artery occlusion (MCAO) in rats. NT-3 significantly reduced the infarct size at 24 h of reperfusion. Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick labeling (TUNEL) staining and immunohistochemical study for caspase-3 and heat shock protein 72 (HSP72) showed that NT-3 treatment decreased the number of cells with DNA fragmentation and caspase-3 and HSP72 expressions. These data suggest that NT-3 protects neuronal cells from ischemic injury, and it is possibly associated with inhibition of DNA fragmentation.

Original languageEnglish
Pages (from-to)211-214
Number of pages4
JournalBrain Research
Volume842
Issue number1
DOIs
Publication statusPublished - Sep 18 1999

Keywords

  • Caspase-3
  • HSP72
  • Ischemia
  • MCAO
  • Neurotrophin-3
  • TUNEL

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Clinical Neurology
  • Developmental Biology

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    Zhang, W. R., Kitagawa, H., Hayashi, T., Sasaki, C., Sakai, K., Warita, H., Shiro, Y., Suenaga, H., Ohmae, H., Tsuji, S., Itoh, T., Nishimura, O., Nagasaki, H., & Abe, K. (1999). Topical application of neurotrophin-3 attenuates ischemic brain injury after transient middle cerebral artery occlusion in rats. Brain Research, 842(1), 211-214. https://doi.org/10.1016/S0006-8993(99)01818-1