TonEBP regulates hyperosmolality-induced arginine vasotocin gene expression in the chick (Gallus domesticus)

Noboru Saito, Mariko Fujii, Kanae Sugiura, Nicoletta Aste, Kiyoshi Shimada

Research output: Contribution to journalArticle

Abstract

Arginine vasotocin (AVT) is expressed mainly in the paraventircular and supraoptic nuclei of the hypothalamus in chicken. This peptide is known to act as an antidiuretic hormone and its gene expression is stimulated by hyperosmolality. However, the transcription factors that regulate the AVT gene expression induced by hyperosmolality are still unknown. In this study, we examined the role of hyper-tonicity enhancer binding protein (TonEBP) in the transcriptional regulation of AVT gene in chicken. TonEBP mRNA expression levels increased at 1 h after salt-loading treatment in the hypothalamus. This increase preceded that in AVT and c-fos mRNA expression. Intracerebroventricular injections of TonEBP antisense oligonucleotides, before the salt-loading treatment, prevented the increase in AVT gene expression. These results, all together, suggest that the transcription factor TonEBP may be involved in the regulation of AVT genes expression in response to a hyperosmotic environment in chicken.

Original languageEnglish
Pages (from-to)334-338
Number of pages5
JournalNeuroscience Letters
Volume468
Issue number3
DOIs
Publication statusPublished - Jan 14 2010
Externally publishedYes

Fingerprint

Vasotocin
Chickens
Carrier Proteins
Gene Expression
Transcription Factors
Salts
Supraoptic Nucleus
Messenger RNA
Antisense Oligonucleotides
Vasopressins
Hypothalamus
Peptides
Injections
Therapeutics
Genes

Keywords

  • AVT
  • Chicken
  • Gene expression
  • Hyperosmolality
  • TonEBP

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

TonEBP regulates hyperosmolality-induced arginine vasotocin gene expression in the chick (Gallus domesticus). / Saito, Noboru; Fujii, Mariko; Sugiura, Kanae; Aste, Nicoletta; Shimada, Kiyoshi.

In: Neuroscience Letters, Vol. 468, No. 3, 14.01.2010, p. 334-338.

Research output: Contribution to journalArticle

Saito, Noboru ; Fujii, Mariko ; Sugiura, Kanae ; Aste, Nicoletta ; Shimada, Kiyoshi. / TonEBP regulates hyperosmolality-induced arginine vasotocin gene expression in the chick (Gallus domesticus). In: Neuroscience Letters. 2010 ; Vol. 468, No. 3. pp. 334-338.
@article{311c2996ce6346d0bd874cf7558645c3,
title = "TonEBP regulates hyperosmolality-induced arginine vasotocin gene expression in the chick (Gallus domesticus)",
abstract = "Arginine vasotocin (AVT) is expressed mainly in the paraventircular and supraoptic nuclei of the hypothalamus in chicken. This peptide is known to act as an antidiuretic hormone and its gene expression is stimulated by hyperosmolality. However, the transcription factors that regulate the AVT gene expression induced by hyperosmolality are still unknown. In this study, we examined the role of hyper-tonicity enhancer binding protein (TonEBP) in the transcriptional regulation of AVT gene in chicken. TonEBP mRNA expression levels increased at 1 h after salt-loading treatment in the hypothalamus. This increase preceded that in AVT and c-fos mRNA expression. Intracerebroventricular injections of TonEBP antisense oligonucleotides, before the salt-loading treatment, prevented the increase in AVT gene expression. These results, all together, suggest that the transcription factor TonEBP may be involved in the regulation of AVT genes expression in response to a hyperosmotic environment in chicken.",
keywords = "AVT, Chicken, Gene expression, Hyperosmolality, TonEBP",
author = "Noboru Saito and Mariko Fujii and Kanae Sugiura and Nicoletta Aste and Kiyoshi Shimada",
year = "2010",
month = "1",
day = "14",
doi = "10.1016/j.neulet.2009.11.027",
language = "English",
volume = "468",
pages = "334--338",
journal = "Neuroscience Letters",
issn = "0304-3940",
publisher = "Elsevier Ireland Ltd",
number = "3",

}

TY - JOUR

T1 - TonEBP regulates hyperosmolality-induced arginine vasotocin gene expression in the chick (Gallus domesticus)

AU - Saito, Noboru

AU - Fujii, Mariko

AU - Sugiura, Kanae

AU - Aste, Nicoletta

AU - Shimada, Kiyoshi

PY - 2010/1/14

Y1 - 2010/1/14

N2 - Arginine vasotocin (AVT) is expressed mainly in the paraventircular and supraoptic nuclei of the hypothalamus in chicken. This peptide is known to act as an antidiuretic hormone and its gene expression is stimulated by hyperosmolality. However, the transcription factors that regulate the AVT gene expression induced by hyperosmolality are still unknown. In this study, we examined the role of hyper-tonicity enhancer binding protein (TonEBP) in the transcriptional regulation of AVT gene in chicken. TonEBP mRNA expression levels increased at 1 h after salt-loading treatment in the hypothalamus. This increase preceded that in AVT and c-fos mRNA expression. Intracerebroventricular injections of TonEBP antisense oligonucleotides, before the salt-loading treatment, prevented the increase in AVT gene expression. These results, all together, suggest that the transcription factor TonEBP may be involved in the regulation of AVT genes expression in response to a hyperosmotic environment in chicken.

AB - Arginine vasotocin (AVT) is expressed mainly in the paraventircular and supraoptic nuclei of the hypothalamus in chicken. This peptide is known to act as an antidiuretic hormone and its gene expression is stimulated by hyperosmolality. However, the transcription factors that regulate the AVT gene expression induced by hyperosmolality are still unknown. In this study, we examined the role of hyper-tonicity enhancer binding protein (TonEBP) in the transcriptional regulation of AVT gene in chicken. TonEBP mRNA expression levels increased at 1 h after salt-loading treatment in the hypothalamus. This increase preceded that in AVT and c-fos mRNA expression. Intracerebroventricular injections of TonEBP antisense oligonucleotides, before the salt-loading treatment, prevented the increase in AVT gene expression. These results, all together, suggest that the transcription factor TonEBP may be involved in the regulation of AVT genes expression in response to a hyperosmotic environment in chicken.

KW - AVT

KW - Chicken

KW - Gene expression

KW - Hyperosmolality

KW - TonEBP

UR - http://www.scopus.com/inward/record.url?scp=72249119749&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=72249119749&partnerID=8YFLogxK

U2 - 10.1016/j.neulet.2009.11.027

DO - 10.1016/j.neulet.2009.11.027

M3 - Article

VL - 468

SP - 334

EP - 338

JO - Neuroscience Letters

JF - Neuroscience Letters

SN - 0304-3940

IS - 3

ER -