Toluene inhalation induced epididymal sperm dysfunction in rats

Atsushi Ono, Kunio Kawashima, Kiyoshi Sekita, Akihiko Hirose, Yukio Ogawa, Minoru Saito, Katsushi Naito, Kazuo Yasuhara, Toyozo Kaneko, Tsuyoshi Furuya, Tohru Inoue, Yuji Kurokawa

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Toluene is a widely abused inhaled solvent. This study was designed to determine whether toluene abuse affects the reproductive functions or general health of males. Seven-week-old male Sprague-Dawley rats were exposed to toluene vapor inhalation (0, 4000, or 6000 ppm; 2 h/day) daily for 5 weeks. Exposure-related suppression of body weight gain and food consumption were observed. Salivation and lacrimation were observed during exposure periods and intensified with repeated exposure. Rats exposed to 6000 ppm toluene had decreased spleen and thymus weights, as well as suppressed lymphocyte counts. In 6000 ppm group, the epididymal sperm counts, sperm motility, sperm quality and in vitro penetrating ability to zona-free hamster eggs were significantly reduced, while no exposure-related changes in the testes weight or spermatogenesis within testes were detected. Tail-less sperm heads were seen within zona-free eggs incubated with sperm from rats exposed to 6000 ppm toluene, but not control rats. No significant changes were observed in serum luteinizing hormone, follicle-stimulating hormone, or testosterone levels following 1 month of exposure to 6000 ppm toluene. These results indicate that high concentrations of toluene may directly target sperm in the epididymis and disrupt sperm maturation.

Original languageEnglish
Pages (from-to)193-205
Number of pages13
JournalToxicology
Volume139
Issue number3
DOIs
Publication statusPublished - Dec 6 1999
Externally publishedYes

Fingerprint

Toluene
Inhalation
Spermatozoa
Rats
Herpes Zoster
Eggs
Testis
Rat control
Sperm Maturation
Sperm Head
Salivation
Weights and Measures
Thymus
Sperm Count
Lymphocytes
Sperm Motility
Epididymis
Lymphocyte Count
Follicle Stimulating Hormone
Spermatogenesis

Keywords

  • Epididymal sperm maturation
  • Fertility
  • Inhalation
  • Toluene abuse

ASJC Scopus subject areas

  • Toxicology

Cite this

Ono, A., Kawashima, K., Sekita, K., Hirose, A., Ogawa, Y., Saito, M., ... Kurokawa, Y. (1999). Toluene inhalation induced epididymal sperm dysfunction in rats. Toxicology, 139(3), 193-205. https://doi.org/10.1016/S0300-483X(99)00120-1

Toluene inhalation induced epididymal sperm dysfunction in rats. / Ono, Atsushi; Kawashima, Kunio; Sekita, Kiyoshi; Hirose, Akihiko; Ogawa, Yukio; Saito, Minoru; Naito, Katsushi; Yasuhara, Kazuo; Kaneko, Toyozo; Furuya, Tsuyoshi; Inoue, Tohru; Kurokawa, Yuji.

In: Toxicology, Vol. 139, No. 3, 06.12.1999, p. 193-205.

Research output: Contribution to journalArticle

Ono, A, Kawashima, K, Sekita, K, Hirose, A, Ogawa, Y, Saito, M, Naito, K, Yasuhara, K, Kaneko, T, Furuya, T, Inoue, T & Kurokawa, Y 1999, 'Toluene inhalation induced epididymal sperm dysfunction in rats', Toxicology, vol. 139, no. 3, pp. 193-205. https://doi.org/10.1016/S0300-483X(99)00120-1
Ono A, Kawashima K, Sekita K, Hirose A, Ogawa Y, Saito M et al. Toluene inhalation induced epididymal sperm dysfunction in rats. Toxicology. 1999 Dec 6;139(3):193-205. https://doi.org/10.1016/S0300-483X(99)00120-1
Ono, Atsushi ; Kawashima, Kunio ; Sekita, Kiyoshi ; Hirose, Akihiko ; Ogawa, Yukio ; Saito, Minoru ; Naito, Katsushi ; Yasuhara, Kazuo ; Kaneko, Toyozo ; Furuya, Tsuyoshi ; Inoue, Tohru ; Kurokawa, Yuji. / Toluene inhalation induced epididymal sperm dysfunction in rats. In: Toxicology. 1999 ; Vol. 139, No. 3. pp. 193-205.
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