Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells

Kimiya Nakamura, Yoshiaki Deyama, Yoshitaka Yoshimura, Kuniaki Suzuki, Manabu Morita

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Double-stranded RNA (dsRNA) and its mimic, polyinosinic acid:polycytidylic acid [poly(I):poly(C)], are recognized by toll-like receptor 3 (TLR3) that induces the production of IFN-β in many cell types. In the present study, we investigated the effects of poly(I):poly(C) on mouse osteoblastic MC3T3-E1 (E1) cells. Poly(I):poly(C) markedly increased IFN-β mRNA level in a dose-dependent manner. The increase in the IFN-β mRNA level was apparent as early as 1 h after adding poly(I):poly(C) to the culture and peaked at 12 h. Stimulation with poly(I):poly(C) enhanced the expression of CXCL10 mRNA and TLR3 in E1 cells. Moreover, poly(I):poly(C) induced tyrosine phosphorylation of the transcription factor STAT1 in E1 cells. An anti-IFN-β neutralizing antibody partially inhibited poly(I):poly(C)-induced CXCL10 mRNA, TLR3 mRNA and STAT1 phosphorylation. These results indicate that osteoblasts secrete IFN-β in response to viral infection and that endogenous IFN-β induces both CXCL10 and TLR3 production via an IFN-α/β receptor-STAT1 pathway. It is suggested that osteoblasts are involved in host defense as well as bone metabolism.

Original languageEnglish
Pages (from-to)771-775
Number of pages5
JournalInternational Journal of Molecular Medicine
Volume19
Issue number5
Publication statusPublished - May 2007
Externally publishedYes

Fingerprint

Toll-Like Receptor 3
Poly C
Poly I-C
Antiviral Agents
Ligands
Messenger RNA
Osteoblasts
Phosphorylation
Poly I
STAT1 Transcription Factor
Double-Stranded RNA
Virus Diseases
Neutralizing Antibodies
Tyrosine
Bone and Bones

Keywords

  • CXCL10
  • Interferon-β
  • Osteoblast
  • Polyinosinic acid:polycytidylic acid
  • Toll-like receptor 3

ASJC Scopus subject areas

  • Genetics

Cite this

Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells. / Nakamura, Kimiya; Deyama, Yoshiaki; Yoshimura, Yoshitaka; Suzuki, Kuniaki; Morita, Manabu.

In: International Journal of Molecular Medicine, Vol. 19, No. 5, 05.2007, p. 771-775.

Research output: Contribution to journalArticle

Nakamura, Kimiya ; Deyama, Yoshiaki ; Yoshimura, Yoshitaka ; Suzuki, Kuniaki ; Morita, Manabu. / Toll-like receptor 3 ligand-induced antiviral response in mouse osteoblastic cells. In: International Journal of Molecular Medicine. 2007 ; Vol. 19, No. 5. pp. 771-775.
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