Toll-like receptor 2 and class B scavenger receptor type I are required for bacterial uptake by trophoblast giant cells

Kenta Watanabe, Eun Kyung Shin, Masanori Hashino, Masato Tachibana, Masahisa Watarai

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Trophoblast giant (TG) cells, components cells of the mouse placenta, exhibit phagocytic activity, and participate in the placental defense system by extracellular bacterial antigen uptake via phagocytosis. However, the bacterial uptake mechanisms by TG cells remain to be entirely understood. In an attempt to understand these mechanisms, in this study, we investigated the pattern recognition receptors (PRRs) involved in phagocytosis by TG cells. PRRs such as Toll-like receptors (TLRs) and scavenger receptors play a critical role in the immune response to bacterial pathogens. Among these, we selected TLR2 and class B scavenger receptor type I (SR-BI) and then evaluated their properties in TG cells. TLR2 and SR-BI expression is higher in TG cells than in trophoblast stem (TS) cells. Although interferon-γ treatment activated bacterial uptake in a concentration-dependent manner, it did not induce TLR2 or SR-BI expression. Depletion of TLR2 and SR-BI by siRNA reduced the bacterial uptake ability of TG cells, which was also affected by treatment with the TLR2 agonist triacylated lipopeptide. These results suggested that the phagocytic activity of TG cells is mediated by both TLR2 and SR-BI.

Original languageEnglish
Pages (from-to)1989-1996
Number of pages8
JournalMolecular Immunology
Volume47
Issue number11-12
DOIs
Publication statusPublished - Jul 2010
Externally publishedYes

Fingerprint

CD36 Antigens
Toll-Like Receptor 2
Trophoblasts
Giant Cells
Pattern Recognition Receptors
Phagocytosis
Lipopeptides
Bacterial Antigens
Scavenger Receptors
Toll-Like Receptors
Cellular Structures
Interferons
Placenta
Small Interfering RNA
Stem Cells

Keywords

  • Class B scavenger receptor type I
  • Phagocytosis
  • Toll-like receptor 2
  • Trophoblast giant cell

ASJC Scopus subject areas

  • Molecular Biology
  • Immunology

Cite this

Toll-like receptor 2 and class B scavenger receptor type I are required for bacterial uptake by trophoblast giant cells. / Watanabe, Kenta; Shin, Eun Kyung; Hashino, Masanori; Tachibana, Masato; Watarai, Masahisa.

In: Molecular Immunology, Vol. 47, No. 11-12, 07.2010, p. 1989-1996.

Research output: Contribution to journalArticle

Watanabe, Kenta ; Shin, Eun Kyung ; Hashino, Masanori ; Tachibana, Masato ; Watarai, Masahisa. / Toll-like receptor 2 and class B scavenger receptor type I are required for bacterial uptake by trophoblast giant cells. In: Molecular Immunology. 2010 ; Vol. 47, No. 11-12. pp. 1989-1996.
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