TMG-chitotriomycin as a probe for the prediction of substrate specificity of β-N-acetylhexosaminidases

Hiroto Shiota, Hiroshi Kanzaki, Tadashi Hatanaka, Teruhiko Nitoda

Research output: Contribution to journalArticlepeer-review

5 Citations (Scopus)

Abstract

TMG-chitotriomycin (1) produced by the actinomycete Streptomyces annulatus NBRC13369 was examined as a probe for the prediction of substrate specificity of β-N-acetylhexosaminidases (HexNAcases). According to the results of inhibition assays, 14 GH20 HexNAcases from various organisms were divided into 1-sensitive and 1-insensitive enzymes. Three representatives of each group were investigated for their substrate specificity. The 1-sensitive HexNAcases hydrolyzed N-acetylchitooligosaccharides but not N-glycan-type oligosaccharides, whereas the 1-insensitive enzymes hydrolyzed N-glycan-type oligosaccharides but not N-acetylchitooligosaccharides, indicating that TMG-chitotriomycin can be used as a molecular probe to distinguish between chitin-degrading HexNAcases and glycoconjugate-processing HexNAcases.

Original languageEnglish
Pages (from-to)29-34
Number of pages6
JournalCarbohydrate Research
Volume375
DOIs
Publication statusPublished - May 21 2013

Keywords

  • GH20 family
  • Inhibitor
  • Physiological function
  • Substrate specificity
  • TMG-chitotriomycin
  • β-N-Acetylhexosaminidase

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Organic Chemistry

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