Tissue-specific organelle DNA degradation mediated by DPD1 Exonuclease

Lay Yin Tang, Wataru Sakamoto

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Organelle DNA in plastids and mitochondria is present in multiple copies and undergoes degradation developmentally. For example, organelle DNA that is detectable cytologically using DNA-fluorescent dye disappears during pollen development. Nevertheless, nucleases involved in this degradation process remain unknown. Our recent study identified the organelle nuclease, DPD1, which has Mg 2+- dependent exonuclease activity in vitro. The discovery of DPD1 emerged from Arabidopsis mutant screening and concomitant isolation of dpd1 mutants that retain organelle DNA in mature pollen. DPD1 is conserved only in angiosperms: not in other photosynthetic organisms. Despite these findings, the physiological significance of organelle DNA degradation during pollen development remains unclear because dpd1 exhibits no apparent defects in pollen viability or in the maternal inheritance of organelle DNA. We discuss a possible role of organelle DNA degradation mediated by DPD1, based on a DPD1 expression profile studied using in silico analyses.

Original languageEnglish
Pages (from-to)1391-1393
Number of pages3
JournalPlant Signaling and Behavior
Volume6
Issue number9
DOIs
Publication statusPublished - Sep 2011

Fingerprint

organelles
degradation
DNA
pollen
nucleases
mutants
plastid DNA
fluorescent dyes
autotrophs
tissues
Angiospermae
inheritance (genetics)
mitochondria
Arabidopsis
viability
screening

Keywords

  • Mitochondria
  • Nuclease
  • Organelle DNAs
  • Plastids
  • Pollen

ASJC Scopus subject areas

  • Plant Science

Cite this

Tissue-specific organelle DNA degradation mediated by DPD1 Exonuclease. / Tang, Lay Yin; Sakamoto, Wataru.

In: Plant Signaling and Behavior, Vol. 6, No. 9, 09.2011, p. 1391-1393.

Research output: Contribution to journalArticle

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