Tissue-specific mRNA expression profiles of drug-metabolizing enzymes and transporters in the cynomolgus monkey

Masuhiro Nishimura, Akiko Koeda, Hiroshi Morikawa, Tetsuo Satoh, Shizuo Narimatsu, Shinsaku Naito

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Pairs of forward and reverse primers and TaqMan probes specific to each of 19 drug-metabolizing enzymes (cytochrome P450s, UDP-glucuronosyltransferases, glutathione S-transferases, and sulfotransferases) and 5 transporters (ABC and SLC transporters) in the cynomolgus monkey were prepared. The expression level of each target mRNA was analyzed in total RNA obtained from three specimens of various cynomolgus monkey tissues (adrenal gland, brain, heart, kidney, large intestine, liver, lung, pancreas, prostate, small intestine, spleen, testis, and thymus) by real-time reverse transcription PCR using an Applied Biosystems 7500 Fast Real-Time PCR System. The data obtained in the present study provide useful information on tissue-specific profiles of the expression of these target mRNAs in the cynomolgus monkey, and the results are expected to be valuable in establishing drug metabolism- and transporter-mediated screening systems using the cynomolgus monkey for the evaluation of new chemical entities in new drug development.

Original languageEnglish
Pages (from-to)139-144
Number of pages6
JournalDrug Metabolism And Pharmacokinetics
Volume24
Issue number2
DOIs
Publication statusPublished - 2009
Externally publishedYes

Keywords

  • Cynomolgus monkey
  • Drug-metabolizing enzymes
  • MRNA expression
  • Quantification
  • Tissue distribution

ASJC Scopus subject areas

  • Pharmacology
  • Pharmaceutical Science
  • Pharmacology (medical)

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