Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans

The role of the C- and N-terminal extensions

Md Ziaul Amin, Tetsuya Bando, Razia Ruksana, Frederick Anokye-Danso, Yasuo Takashima, Yasuji Sakube, Hiroaki Kagawa

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of this study is to investigate the function of the C-terminal extension of three troponin I isoforms, that are unique to the body wall muscles of Caenorhabditis elegans and to understand the molecular interactions within the TN complex between troponin I with troponin C/T, and tropomyosin. We constructed several expression vectors to generate recombinant proteins of three body wall and one pharyngeal troponin I isoforms in Escherichia coli. Protein overlay assays and Western blot analyses were performed using antibodies. We demonstrated that pharyngeal TNI-4 interacted with only the pharyngeal isoforms of troponin C/T and tropomyosin. In contrast, the body wall TNI-2 bound both the body wall and pharyngeal isoforms of these components. Similar to other invertebrates, the N-terminus of troponin I contributes to interactions with troponin C. Full-length troponin I was essential for interactions with tropomyosin isoforms. Deletion of the C-terminal extension had no direct effect on the binding of the body wall troponin I to other muscle thin filament troponin C/T and tropomyosin isoforms.

Original languageEnglish
Pages (from-to)456-465
Number of pages10
JournalBiochimica et Biophysica Acta - Proteins and Proteomics
Volume1774
Issue number4
DOIs
Publication statusPublished - Apr 2007

Fingerprint

Tropomyosin
Troponin I
Troponin C
Protein Isoforms
Tissue
Troponin T
Muscle
Muscles
Molecular interactions
Caenorhabditis elegans
Invertebrates
Recombinant Proteins
Escherichia coli
Assays
Western Blotting
Antibodies
Proteins

Keywords

  • C-terminal extension
  • Caenorhabditis elegans
  • Tropomyosin
  • Troponin C
  • Troponin I
  • Troponin T

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Genetics

Cite this

Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans : The role of the C- and N-terminal extensions. / Amin, Md Ziaul; Bando, Tetsuya; Ruksana, Razia; Anokye-Danso, Frederick; Takashima, Yasuo; Sakube, Yasuji; Kagawa, Hiroaki.

In: Biochimica et Biophysica Acta - Proteins and Proteomics, Vol. 1774, No. 4, 04.2007, p. 456-465.

Research output: Contribution to journalArticle

Amin, Md Ziaul ; Bando, Tetsuya ; Ruksana, Razia ; Anokye-Danso, Frederick ; Takashima, Yasuo ; Sakube, Yasuji ; Kagawa, Hiroaki. / Tissue-specific interactions of TNI isoforms with other TN subunits and tropomyosins in C. elegans : The role of the C- and N-terminal extensions. In: Biochimica et Biophysica Acta - Proteins and Proteomics. 2007 ; Vol. 1774, No. 4. pp. 456-465.
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