Tissue-specific expression of human provirus ERV3 mRNA in human placenta: Two of the three ERV3 mRNAs contain human cellular sequences

Nobuyuki Kato, S. Pfeifer-Ohlsson, M. Kato, E. Larsson, J. Rydnert, M. Cohen

Research output: Contribution to journalArticle

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Abstract

Three polyadenylated RNAs, 9, 7.3, and 3.5 kilobases long, of a human endogenous retrovirus, ERV3, are abundant in human placental chorion, representing about 0.03 to 0.05% of the total mRNA. We characterized the structure of these mRNAs by Northern blot and S1 nuclease mapping analyses. We found that all three RNAs were spliced mRNAs that lacked 5.9 kilobases of proviral sequence, including the gag gene and most of the pol gene. In contrast to the transcription pattern usual for other retroviruses, the transcription pattern of the ERV3 provirus did not include a genome-length mRNA. All three of the ERV3 mRNAs initiated transcription at the same point in the 5' long terminal repeat (LTR) and contained identical splice junctions in the provirus. The 3.5-kilobase RNA was a typical subgenomic proviral mRNA, with its polyadenylation site in the 3' LTC. The two larger ERV3 mRNAs, however, extended through the polyadenylation site in the 3' LTR and were spliced at a second position approximately 370 nucleotides downstream from the 3' LTR. This finding suggests that when the ERV3 retrovirus integrated at this genomic locus in an ancester of humans, it integrated within or adjacent to a cellular gene.

Original languageEnglish
Pages (from-to)2182-2191
Number of pages10
JournalJournal of Virology
Volume61
Issue number7
Publication statusPublished - 1987
Externally publishedYes

Fingerprint

Retroviridae
proviruses
Proviruses
terminal repeat sequences
placenta
Placenta
transcription (genetics)
Messenger RNA
RNA
Terminal Repeat Sequences
chorion
genes
nucleases
messenger RNA
Northern blotting
Polyadenylation
nucleotides
genomics
loci
genome

ASJC Scopus subject areas

  • Immunology

Cite this

Tissue-specific expression of human provirus ERV3 mRNA in human placenta : Two of the three ERV3 mRNAs contain human cellular sequences. / Kato, Nobuyuki; Pfeifer-Ohlsson, S.; Kato, M.; Larsson, E.; Rydnert, J.; Cohen, M.

In: Journal of Virology, Vol. 61, No. 7, 1987, p. 2182-2191.

Research output: Contribution to journalArticle

Kato, N, Pfeifer-Ohlsson, S, Kato, M, Larsson, E, Rydnert, J & Cohen, M 1987, 'Tissue-specific expression of human provirus ERV3 mRNA in human placenta: Two of the three ERV3 mRNAs contain human cellular sequences', Journal of Virology, vol. 61, no. 7, pp. 2182-2191.
Kato, Nobuyuki ; Pfeifer-Ohlsson, S. ; Kato, M. ; Larsson, E. ; Rydnert, J. ; Cohen, M. / Tissue-specific expression of human provirus ERV3 mRNA in human placenta : Two of the three ERV3 mRNAs contain human cellular sequences. In: Journal of Virology. 1987 ; Vol. 61, No. 7. pp. 2182-2191.
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