Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans

Razia Ruksana, Kazuki Kuroda, Hiromi Terami, Tetsuya Bando, Shun Kitaoka, Tomohide Takaya, Yasuji Sakube, Hiroaki Kagawa

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Gene duplication is a major genetic event that can produce multiple protein isoforms. Comparative sequence and functional analysis of related gene products can provide insights into protein family evolution. To characterize the Caenorhabditis elegans troponin I family, we analyzed gene structures, tissue expression patterns and RNAi phenotypes of four troponin I isoforms. Tissue expression patterns were determined using lacZ/gfp/rfp reporter gene assays. The tni-1, tni-2/unc-27 and tni-3 genes, each encoding a troponin I isoform, are uniquely expressed in body wall, vulval and anal muscles but at different levels; tni-4 was expressed solely in the pharynx. Expressing tni-1 and -2 gene RNAi caused motility defects similar to unc-27 (e155) mutant, a tni-2 null allele. The tni-3 RNAi expression produced egg laying defects while the tni-4 RNAi caused arrest at gastrulation. Overlay analyses were used to assay interactions between the troponin I and two troponin C isoforms. The three body wall troponin I isoforms interacted with body wall and pharyngeal troponin C isoforms; TNI-4 interacted only with pharyngeal troponin C. Our results suggest the body wall genes have evolved following duplication of the pharynx gene and provide important data about gene duplication and functional differentiation of nematode troponin I isoforms.

Original languageEnglish
Pages (from-to)261-276
Number of pages16
JournalGenes to Cells
Volume10
Issue number3
DOIs
Publication statusPublished - Mar 2005

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Troponin C
Troponin I
Caenorhabditis elegans
Protein Isoforms
RNA Interference
Gene Duplication
Genes
Pharynx
Gastrulation
Reporter Genes
Ovum
Sequence Analysis
Alleles
Phenotype
Muscles

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

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Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans. / Ruksana, Razia; Kuroda, Kazuki; Terami, Hiromi; Bando, Tetsuya; Kitaoka, Shun; Takaya, Tomohide; Sakube, Yasuji; Kagawa, Hiroaki.

In: Genes to Cells, Vol. 10, No. 3, 03.2005, p. 261-276.

Research output: Contribution to journalArticle

Ruksana, Razia ; Kuroda, Kazuki ; Terami, Hiromi ; Bando, Tetsuya ; Kitaoka, Shun ; Takaya, Tomohide ; Sakube, Yasuji ; Kagawa, Hiroaki. / Tissue expression of four troponin I genes and their molecular interactions with two troponin C isoforms in Caenorhabditis elegans. In: Genes to Cells. 2005 ; Vol. 10, No. 3. pp. 261-276.
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