Tissue culture and transformation of Oenothera biennis

D. Pavingerová; I. Gális; M. Ondřej

doi:10.1007/BF02879628

Tissue culture and transformation of Oenothera biennis

D. Pavingerová, I. Gális, M. Ondřej

Research output: Contribution to journal › Article › peer-review

6 Citations (Scopus)

Abstract

Five cultivars of Oenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transformed by Agrobacterium tumefaciens strain which introduces into the plant genome kanamycin resistance gene and the T-DNA ipt gene which causes increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, branched shoots with small modified leaves. Roots appeared rarely in later subcultivations of some teratomous clones.

Original language	English
Pages (from-to)	27-32
Number of pages	6
Journal	Biologia Plantarum
Volume	38
Issue number	1
DOIs	https://doi.org/10.1007/BF02879628
Publication status	Published - Jan 1 1996
Externally published	Yes

Keywords

Agrobacterium tumefaciens
Cytokinins
Regeneration

ASJC Scopus subject areas

Plant Science
Horticulture

Access to Document

10.1007/BF02879628

Fingerprint Dive into the research topics of 'Tissue culture and transformation of Oenothera biennis'. Together they form a unique fingerprint.

Cite this

@article{e5ad16b5509b405d84cebc970597b758,

title = "Tissue culture and transformation of Oenothera biennis",

abstract = "Five cultivars of Oenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transformed by Agrobacterium tumefaciens strain which introduces into the plant genome kanamycin resistance gene and the T-DNA ipt gene which causes increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, branched shoots with small modified leaves. Roots appeared rarely in later subcultivations of some teratomous clones.",

keywords = "Agrobacterium tumefaciens, Cytokinins, Regeneration",

author = "D. Pavingerov{\'a} and I. G{\'a}lis and M. Ond{\v r}ej",

year = "1996",

month = jan,

day = "1",

doi = "10.1007/BF02879628",

language = "English",

volume = "38",

pages = "27--32",

journal = "Biologia Plantarum",

issn = "0006-3134",

publisher = "Springer Netherlands",

number = "1",

}

TY - JOUR

T1 - Tissue culture and transformation of Oenothera biennis

AU - Pavingerová, D.

AU - Gális, I.

AU - Ondřej, M.

PY - 1996/1/1

Y1 - 1996/1/1

N2 - Five cultivars of Oenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transformed by Agrobacterium tumefaciens strain which introduces into the plant genome kanamycin resistance gene and the T-DNA ipt gene which causes increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, branched shoots with small modified leaves. Roots appeared rarely in later subcultivations of some teratomous clones.

AB - Five cultivars of Oenothera biennis have been tested for callogenesis and organogenesis on different media. The cultivar CV3 has been transformed by Agrobacterium tumefaciens strain which introduces into the plant genome kanamycin resistance gene and the T-DNA ipt gene which causes increased levels of cytokinins. Transformed tissues showed elevated levels of cytokinins and grew as teratomas forming clumps of short, branched shoots with small modified leaves. Roots appeared rarely in later subcultivations of some teratomous clones.

KW - Agrobacterium tumefaciens

KW - Cytokinins

KW - Regeneration

UR - http://www.scopus.com/inward/record.url?scp=0030026125&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030026125&partnerID=8YFLogxK

U2 - 10.1007/BF02879628

DO - 10.1007/BF02879628

M3 - Article

AN - SCOPUS:0030026125

VL - 38

SP - 27

EP - 32

JO - Biologia Plantarum

JF - Biologia Plantarum

SN - 0006-3134

IS - 1

ER -

Tissue culture and transformation of Oenothera biennis

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Cite this