TIMP-1 production by human scleral fibroblast decreases in response to cyclic mechanical stretching

Akihiro Yamaoka, Toshihiko Matsuo, Fumio Shiraga, Hiroshi Ohtsuki

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22 Citations (Scopus)


Purpose: The effect of mechanical stretching was examined on cultured scleral fibroblasts of the human eye in order to observe changes in their production of TIMP (tissue inhibitor of metalloproteinase)-1, MMP (matrix metalloproteinase)-1 and -2 in response to physiological strain. Methods: Human scleral fibroblasts were cultured from scleral tissue resected during foveal translocation surgery. The fibroblasts in near confluency were exposed to mechanical stretching of the bottom of a 6-cm Petri dish at the maximum magnitude of 4500 microstrain and at a cycle of 30 s for 72 h. TIMP-1, MMP-1 and MMP-2 levels in the medium following 24, 48 and 72 h of cyclic stretching were measured by enzyme immunoassay. Results: The growth of scleral fibroblasts during the 72-hour period of stretching did not show a significant difference from that of non-stretched control fibroblasts. Scleral fibroblasts in the stretched group produced a significantly smaller amount of TIMP-1 at 72 h after stretching, compared with nonstretched control (p = 0.0353, Student t-test). The levels of MMP-1 and MMP-2 produced by scleral fibroblasts were not significantly different between the stretched group and nonstretched group. Conclusion: The production of TIMP-1 by human scleral fibroblasts was suppressed by cyclic mechanical stretching. Mechanical strain would be one factor to regulate the homeostasis of extracellular matrix in the sclera.

Original languageEnglish
Pages (from-to)98-101
Number of pages4
JournalOphthalmic Research
Issue number2
Publication statusPublished - Mar 28 2001


  • Human scleral fibroblast
  • MMP-1
  • MMP-2
  • Mechanical stretching
  • TIMP-1

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience


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