Timing of the G1/S transition in tobacco pollen vegetative cells as a primary step towards androgenesis in vitro

Masaharu Kyo, Ai Nagano, Naoki Yamaji, Yuhki Hashimoto

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Key message: Mid-bicellular pollen vegetative cells in tobacco escape from G1 arrest and proceed to the G1/S transition towards androgenesis within 1 day under glutamine starvation conditions in vitro. In the Nicotiana tabacum pollen culture system, immature pollen grains at the mid-bicellular stage can mature in the presence of glutamine; however, if glutamine is absent, they deviate from their native cell fate in a few days. The glutamine-starved pollen grains cannot undergo maturation, even when supplied with glutamine later. Instead, they undergo cell division towards androgenesis slowly within 10 days in a medium containing appropriate nutrients. During the culture period, they ought to escape from G1 arrest to proceed into S phase as the primary step towards androgenesis. However, this event has not been experimentally confirmed. Here, we demonstrated that the pollen vegetative cells proceeded to the G1/S transition within approximately 15-36 h after the start of culture. These results were obtained by analyzing transgenic pollen possessing a fusion gene encoding nuclear-localizing GFP under the control of an E2F motif-containing promoter isolated from a gene encoding one of DNA replication licensing factors. Observations using a 5-ethynyl-2′-deoxyuridine DNA labeling and detection technique uncovered that the G1/S transition was soon followed by S phase. These hallmarks of vegetative cells undergoing dedifferentiation give us new insights into upstream events causing the G1/S transition and also provide a novel strategy to increase the frequency of the androgenic response in tobacco and other species, including recalcitrants.

Original languageEnglish
Pages (from-to)1595-1606
Number of pages12
JournalPlant Cell Reports
Volume33
Issue number9
DOIs
Publication statusPublished - 2014

Fingerprint

androgenesis
vegetative cells
Pollen
Tobacco
Glutamine
glutamine
tobacco
pollen
S Phase
interphase
Cell Dedifferentiation
cell dedifferentiation
gene fusion
Gene Fusion
DNA replication
Licensure
Starvation
DNA Replication
Cell Division
Nicotiana tabacum

Keywords

  • Androgenesis
  • Dedifferentiation
  • E2F motif
  • G1/S transition
  • Nicotiana tabacum
  • Pollen embryogenesis

ASJC Scopus subject areas

  • Plant Science
  • Agronomy and Crop Science
  • Medicine(all)

Cite this

Timing of the G1/S transition in tobacco pollen vegetative cells as a primary step towards androgenesis in vitro. / Kyo, Masaharu; Nagano, Ai; Yamaji, Naoki; Hashimoto, Yuhki.

In: Plant Cell Reports, Vol. 33, No. 9, 2014, p. 1595-1606.

Research output: Contribution to journalArticle

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