Timeless Plays an Important Role in Compound Eye-Dependent Photic Entrainment of the Circadian Rhythm in the Cricket Gryllus bimaculatus

Yoshiyuki Moriyama, Kazuki Takeuchi, Tsugumichi Shinohara, Koichi Miyagawa, Mirai Matsuka, Taishi Yoshii, Kenji Tomioka

Research output: Contribution to journalArticlepeer-review

Abstract

The light cycle is the most powerful Zeitgeber entraining the circadian clock in most organisms. Insects use CRYPTOCHROMEs (CRYs) and/or the compound eye for the light perception necessary for photic entrainment. The molecular mechanism underlying CRY-dependent entrainment is well understood, while that of the compound eye-dependent entrainment remains to be elucidated. Using molecular and behavioral experiments, we investigated the role of timeless (tim) in the photic entrainment mechanism in the cricket Gryllus bimaculatus. RNA interference of tim (timRNAi) disrupted the entrainment or prolonged the transients for resynchronization to phase-delayed light-dark cycles. The treatment reduced the magnitude of phase delay caused by delayed light-off, but augmented advance shifts caused by light exposure at late night. TIM protein levels showed daily cycling with an increase during the night and reduction by light exposure at both early and late night. These results suggest that tim plays a critical role in the entrainment to delayed light cycles.

Original languageEnglish
Pages (from-to)397-405
Number of pages9
JournalZoological science
Volume39
Issue number4
DOIs
Publication statusPublished - Aug 1 2022

Keywords

  • circadian clock
  • compound eye
  • cricket
  • entrainment
  • phase shifts
  • RNAi
  • tim

ASJC Scopus subject areas

  • Medicine(all)

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