Time-resolved studies of metalloproteins using X-ray free electron laser radiation at SACLA

Michihiro Suga, Atsuhiro Shimada, Fusamichi Akita, Jian Ren Shen, Takehiko Tosha, Hiroshi Sugimoto

Research output: Contribution to journalArticle

Abstract

Background: The invention of the X-ray free-electron laser (XFEL) has provided unprecedented new opportunities for structural biology. The advantage of XFEL is an intense pulse of X-rays and a very short pulse duration (<10 fs) promising a damage-free and time-resolved crystallography approach. Scope of review: Recent time-resolved crystallographic analyses in XFEL facility SACLA are reviewed. Specifically, metalloproteins involved in the essential reactions of bioenergy conversion including photosystem II, cytochrome c oxidase and nitric oxide reductase are described. Major conclusions: XFEL with pump-probe techniques successfully visualized the process of the reaction and the dynamics of a protein. Since the active center of metalloproteins is very sensitive to the X-ray radiation, damage-free structures obtained by XFEL are essential to draw mechanistic conclusions. Methods and tools for sample delivery and reaction initiation are key for successful measurement of the time-resolved data. General significance: XFEL is at the center of approaches to gain insight into complex mechanism of structural dynamics and the reactions catalyzed by biological macromolecules. Further development has been carried out to expand the application of time-resolved X-ray crystallography. This article is part of a Special Issue entitled Novel measurement techniques for visualizing ‘live’ protein molecules.

Original languageEnglish
Article number129466
JournalBiochimica et Biophysica Acta - General Subjects
Volume1864
Issue number2
DOIs
Publication statusPublished - Feb 2020

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Metalloproteins
X ray lasers
Time and motion study
Free electron lasers
Laser radiation
Lasers
X-Rays
Electrons
Radiation
X rays
Photosystem II Protein Complex
Crystallography
X ray crystallography
Radiation damage
Structural dynamics
Patents and inventions
Electron Transport Complex IV
Macromolecules
Conversion Disorder
Laser pulses

Keywords

  • Heme
  • Metalloproteins
  • Proton pump
  • Radiation damage
  • Serial femtosecond crystallography
  • X-ray free-electron laser

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology

Cite this

Time-resolved studies of metalloproteins using X-ray free electron laser radiation at SACLA. / Suga, Michihiro; Shimada, Atsuhiro; Akita, Fusamichi; Shen, Jian Ren; Tosha, Takehiko; Sugimoto, Hiroshi.

In: Biochimica et Biophysica Acta - General Subjects, Vol. 1864, No. 2, 129466, 02.2020.

Research output: Contribution to journalArticle

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