Time-dependent image changes after ethanol injection into the pancreas: An experimental study using a porcine model

Kazuyuki Matsumoto, Hironari Katou, Koichiro Tsutsumi, Soichiro Fushimi, Masaya Iwamuro, Shinsuke Oda, Sho Mizukawa, Yutaka Akimoto, Daisuke Uchida, Takeshi Tomoda, Naoki Yamamoto, Shigeru Horiguchi, Hiroyuki Okada

Research output: Contribution to journalArticle

Abstract

Background: Ethanol, a commonly available agent, has been used to successfully ablate cystic and solid lesions in the pancreas. The aim of this study is to investigate the effects of an ethanol injection into the porcine pancreas and observe the time-dependent image changes in the pancreatic parenchyma. Methods: Pure ethanol was injected into the pancreatic tail using a 25-gauge EUS needle with direct ultrasound guidance under celiotomy: 1 mL and 2 mL were injected, respectively. The abdomen was closed after the injection. MRI was performed before the procedure, immediately after, and on postoperative day (POD) seven. Blood samples were taken before the procedure and on PODs one, three, five, and seven. The pigs were euthanised on POD seven. Results: Immediately after the injection, linear high signal areas in the pancreatic tail on T2 and rounded speckled high signal areas on DWI images were detected in both animals, measuring 35 × 32 mm in the 1 mL injected pig and 42 × 38mm in the 2 mL injected pig. After POD seven, rounded high signal areas were noted on T2 images, measuring 22 × 18 mm and 36 × 28 mm respectively. On POD one, the 1 mL injected animal had a 53% elevation in serum amylase while the 2 mL injected animal had a 66% elevation. Histologically, cystic and necrotic changes in the parenchyma were observed, measuring 23 × 22 mm and 40 × 35 mm respectively. Conclusions: Our results, which are limited to normal pancreas, suggested that a 1 mL injection caused localised changes within the pancreas while a 2 mL injection induced more widespread changes beyond the pancreas. The effective area of ethanol was widespread immediately after injection, and then the area was reduced with cystic and necrosis changes.

Original languageEnglish
Article number663
Journalecancermedicalscience
Volume10
DOIs
Publication statusPublished - Aug 15 2016

Fingerprint

Pancreas
Ethanol
Swine
Injections
Tail
Amylases
Abdomen
Needles
Necrosis
Serum

Keywords

  • Ethanol injection
  • Magnetic resonance imaging
  • Pancreas
  • Porcine model
  • Ultrasound

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

@article{201a6b7b1de04adca3f52a29870cd9fe,
title = "Time-dependent image changes after ethanol injection into the pancreas: An experimental study using a porcine model",
abstract = "Background: Ethanol, a commonly available agent, has been used to successfully ablate cystic and solid lesions in the pancreas. The aim of this study is to investigate the effects of an ethanol injection into the porcine pancreas and observe the time-dependent image changes in the pancreatic parenchyma. Methods: Pure ethanol was injected into the pancreatic tail using a 25-gauge EUS needle with direct ultrasound guidance under celiotomy: 1 mL and 2 mL were injected, respectively. The abdomen was closed after the injection. MRI was performed before the procedure, immediately after, and on postoperative day (POD) seven. Blood samples were taken before the procedure and on PODs one, three, five, and seven. The pigs were euthanised on POD seven. Results: Immediately after the injection, linear high signal areas in the pancreatic tail on T2 and rounded speckled high signal areas on DWI images were detected in both animals, measuring 35 × 32 mm in the 1 mL injected pig and 42 × 38mm in the 2 mL injected pig. After POD seven, rounded high signal areas were noted on T2 images, measuring 22 × 18 mm and 36 × 28 mm respectively. On POD one, the 1 mL injected animal had a 53{\%} elevation in serum amylase while the 2 mL injected animal had a 66{\%} elevation. Histologically, cystic and necrotic changes in the parenchyma were observed, measuring 23 × 22 mm and 40 × 35 mm respectively. Conclusions: Our results, which are limited to normal pancreas, suggested that a 1 mL injection caused localised changes within the pancreas while a 2 mL injection induced more widespread changes beyond the pancreas. The effective area of ethanol was widespread immediately after injection, and then the area was reduced with cystic and necrosis changes.",
keywords = "Ethanol injection, Magnetic resonance imaging, Pancreas, Porcine model, Ultrasound",
author = "Kazuyuki Matsumoto and Hironari Katou and Koichiro Tsutsumi and Soichiro Fushimi and Masaya Iwamuro and Shinsuke Oda and Sho Mizukawa and Yutaka Akimoto and Daisuke Uchida and Takeshi Tomoda and Naoki Yamamoto and Shigeru Horiguchi and Hiroyuki Okada",
year = "2016",
month = "8",
day = "15",
doi = "10.3332/ecancer.2016.663",
language = "English",
volume = "10",
journal = "ecancermedicalscience",
issn = "1754-6605",
publisher = "Cancer Intellilgence",

}

TY - JOUR

T1 - Time-dependent image changes after ethanol injection into the pancreas

T2 - An experimental study using a porcine model

AU - Matsumoto, Kazuyuki

AU - Katou, Hironari

AU - Tsutsumi, Koichiro

AU - Fushimi, Soichiro

AU - Iwamuro, Masaya

AU - Oda, Shinsuke

AU - Mizukawa, Sho

AU - Akimoto, Yutaka

AU - Uchida, Daisuke

AU - Tomoda, Takeshi

AU - Yamamoto, Naoki

AU - Horiguchi, Shigeru

AU - Okada, Hiroyuki

PY - 2016/8/15

Y1 - 2016/8/15

N2 - Background: Ethanol, a commonly available agent, has been used to successfully ablate cystic and solid lesions in the pancreas. The aim of this study is to investigate the effects of an ethanol injection into the porcine pancreas and observe the time-dependent image changes in the pancreatic parenchyma. Methods: Pure ethanol was injected into the pancreatic tail using a 25-gauge EUS needle with direct ultrasound guidance under celiotomy: 1 mL and 2 mL were injected, respectively. The abdomen was closed after the injection. MRI was performed before the procedure, immediately after, and on postoperative day (POD) seven. Blood samples were taken before the procedure and on PODs one, three, five, and seven. The pigs were euthanised on POD seven. Results: Immediately after the injection, linear high signal areas in the pancreatic tail on T2 and rounded speckled high signal areas on DWI images were detected in both animals, measuring 35 × 32 mm in the 1 mL injected pig and 42 × 38mm in the 2 mL injected pig. After POD seven, rounded high signal areas were noted on T2 images, measuring 22 × 18 mm and 36 × 28 mm respectively. On POD one, the 1 mL injected animal had a 53% elevation in serum amylase while the 2 mL injected animal had a 66% elevation. Histologically, cystic and necrotic changes in the parenchyma were observed, measuring 23 × 22 mm and 40 × 35 mm respectively. Conclusions: Our results, which are limited to normal pancreas, suggested that a 1 mL injection caused localised changes within the pancreas while a 2 mL injection induced more widespread changes beyond the pancreas. The effective area of ethanol was widespread immediately after injection, and then the area was reduced with cystic and necrosis changes.

AB - Background: Ethanol, a commonly available agent, has been used to successfully ablate cystic and solid lesions in the pancreas. The aim of this study is to investigate the effects of an ethanol injection into the porcine pancreas and observe the time-dependent image changes in the pancreatic parenchyma. Methods: Pure ethanol was injected into the pancreatic tail using a 25-gauge EUS needle with direct ultrasound guidance under celiotomy: 1 mL and 2 mL were injected, respectively. The abdomen was closed after the injection. MRI was performed before the procedure, immediately after, and on postoperative day (POD) seven. Blood samples were taken before the procedure and on PODs one, three, five, and seven. The pigs were euthanised on POD seven. Results: Immediately after the injection, linear high signal areas in the pancreatic tail on T2 and rounded speckled high signal areas on DWI images were detected in both animals, measuring 35 × 32 mm in the 1 mL injected pig and 42 × 38mm in the 2 mL injected pig. After POD seven, rounded high signal areas were noted on T2 images, measuring 22 × 18 mm and 36 × 28 mm respectively. On POD one, the 1 mL injected animal had a 53% elevation in serum amylase while the 2 mL injected animal had a 66% elevation. Histologically, cystic and necrotic changes in the parenchyma were observed, measuring 23 × 22 mm and 40 × 35 mm respectively. Conclusions: Our results, which are limited to normal pancreas, suggested that a 1 mL injection caused localised changes within the pancreas while a 2 mL injection induced more widespread changes beyond the pancreas. The effective area of ethanol was widespread immediately after injection, and then the area was reduced with cystic and necrosis changes.

KW - Ethanol injection

KW - Magnetic resonance imaging

KW - Pancreas

KW - Porcine model

KW - Ultrasound

UR - http://www.scopus.com/inward/record.url?scp=84990181149&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84990181149&partnerID=8YFLogxK

U2 - 10.3332/ecancer.2016.663

DO - 10.3332/ecancer.2016.663

M3 - Article

AN - SCOPUS:84990181149

VL - 10

JO - ecancermedicalscience

JF - ecancermedicalscience

SN - 1754-6605

M1 - 663

ER -