Abstract
The time-dependent tumor necrosis factor-alpha (TNF)-induced changes in the plasma membrane lipid bilayer have been investigated in K562 cells, a human chronic myelocytic leukemia cell line, using spin label electron spin resonance techniques. Treatment of K562 cells in suspension culture with TNF for as long a time as 6 h caused an increase in cell membrane fluidity (CMF) and returned to the level of control cells at 12 h. But, conversely, by 24 h after beginning of treatment, the rigidity of CMF was increased and remained so for at least 2 days. The change of TNF-induced CMF in K562 cells was biphasic, but not in Daudi cells, a human malignant lymphoma cell line. Thus, the TNF-induced perturbation of membrane structure is specific. The late increase in membrane rigidity may have a different underlying mechanism from that of the early change. While the early and transient change probably is related to signal generation and transmission, the later and persistent change may be an aspect of the phenotype of TNF-treated cells and may reflect changes in the plasma membrane-cytoplasmic complex. In addition, membrane changes induced by TNF have an ultrastructural correlate.
| Original language | English |
|---|---|
| Pages (from-to) | 422-424 |
| Number of pages | 3 |
| Journal | Biotherapy |
| Volume | 9 |
| Issue number | 3 |
| Publication status | Published - 1995 |
| Externally published | Yes |
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Keywords
- cell membrane fluidity
- tumor necrosis factor-alpha
- ultrastructure
ASJC Scopus subject areas
- Cancer Research
- Immunology and Allergy
Cite this
Time-dependent cell membrane fluidity changes induced by tumor necrosis factor-alpha and transmission electron microscopy morphologic investigation. / Marutaka, M.; Iwagaki, H.; Mizukawa, K.; Gochi, A.; Tanaka, N.; Orita, K.
In: Biotherapy, Vol. 9, No. 3, 1995, p. 422-424.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Time-dependent cell membrane fluidity changes induced by tumor necrosis factor-alpha and transmission electron microscopy morphologic investigation
AU - Marutaka, M.
AU - Iwagaki, H.
AU - Mizukawa, K.
AU - Gochi, A.
AU - Tanaka, N.
AU - Orita, K.
PY - 1995
Y1 - 1995
N2 - The time-dependent tumor necrosis factor-alpha (TNF)-induced changes in the plasma membrane lipid bilayer have been investigated in K562 cells, a human chronic myelocytic leukemia cell line, using spin label electron spin resonance techniques. Treatment of K562 cells in suspension culture with TNF for as long a time as 6 h caused an increase in cell membrane fluidity (CMF) and returned to the level of control cells at 12 h. But, conversely, by 24 h after beginning of treatment, the rigidity of CMF was increased and remained so for at least 2 days. The change of TNF-induced CMF in K562 cells was biphasic, but not in Daudi cells, a human malignant lymphoma cell line. Thus, the TNF-induced perturbation of membrane structure is specific. The late increase in membrane rigidity may have a different underlying mechanism from that of the early change. While the early and transient change probably is related to signal generation and transmission, the later and persistent change may be an aspect of the phenotype of TNF-treated cells and may reflect changes in the plasma membrane-cytoplasmic complex. In addition, membrane changes induced by TNF have an ultrastructural correlate.
AB - The time-dependent tumor necrosis factor-alpha (TNF)-induced changes in the plasma membrane lipid bilayer have been investigated in K562 cells, a human chronic myelocytic leukemia cell line, using spin label electron spin resonance techniques. Treatment of K562 cells in suspension culture with TNF for as long a time as 6 h caused an increase in cell membrane fluidity (CMF) and returned to the level of control cells at 12 h. But, conversely, by 24 h after beginning of treatment, the rigidity of CMF was increased and remained so for at least 2 days. The change of TNF-induced CMF in K562 cells was biphasic, but not in Daudi cells, a human malignant lymphoma cell line. Thus, the TNF-induced perturbation of membrane structure is specific. The late increase in membrane rigidity may have a different underlying mechanism from that of the early change. While the early and transient change probably is related to signal generation and transmission, the later and persistent change may be an aspect of the phenotype of TNF-treated cells and may reflect changes in the plasma membrane-cytoplasmic complex. In addition, membrane changes induced by TNF have an ultrastructural correlate.
KW - cell membrane fluidity
KW - tumor necrosis factor-alpha
KW - ultrastructure
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M3 - Article
AN - SCOPUS:0028935814
VL - 9
SP - 422
EP - 424
JO - Biotherapy
JF - Biotherapy
SN - 0914-2223
IS - 3
ER -