Time-dependent and regional expression of GABA transporter mRNAs following amygdala-kindled seizures in rats

Toru Hirao, Kiyoshi Morimoto, Yoshitaka Yamamoto, Takemi Watanabe, Hitoshi Sato, Keiko Sato, Soichiro Sato, Norihito Yamada, Koichi Tanaka, Hiroshi Suwaki

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Abstract

To investigate the role played by GABA transporters in epileptic seizures, we examined time-dependent and regional changes in expression of GAT-1 and GAT-3 GABA transporter mRNA in amygdala-kindled rat brain using an in situ hybridization method. GAT-1 mRNA was significantly increased bilaterally in the hippocampal dentate gyrus (111-116%) at 1 h after kindled generalized seizures. GAT-1 mRNA was also significantly increased bilaterally in the hippocampal subfields (CA1-4 and dentate gyrus [110-117%]) at 4 h after kindled seizures. There were no significant changes in GAT-1 mRNA level in the amygdalar nuclei, pyriform cortex or cerebral cortex either ipsilaterally or contralaterally at any time after kindled seizures. In contrast, GAT-3 mRNA was significantly increased bilaterally in the amygdalar nuclei and in the contralateral pyriform cortex and cerebral cortex I h after seizures. Since all these changes returned to control levels by 8 or 24 h after kindled seizures, the increases in GABA transporter mRNA appeared to be transient responses to seizure activity. These findings indicate that GAT-1 subtype transporter is specifically involved in seizure activity in the hippocampus, while GAT-3 subtype transporter is mainly involved in seizure activity in the amygdalar nuclei and pyriform cortex following amygdala-kindled generalized seizures.

Original languageEnglish
Pages (from-to)49-55
Number of pages7
JournalMolecular Brain Research
Volume54
Issue number1
DOIs
Publication statusPublished - Feb 1 1998

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Keywords

  • Amygdala
  • GABA transporter
  • Hippocampus
  • In situ hybridization
  • Kindling
  • Rat
  • mRNA

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

Cite this

Hirao, T., Morimoto, K., Yamamoto, Y., Watanabe, T., Sato, H., Sato, K., Sato, S., Yamada, N., Tanaka, K., & Suwaki, H. (1998). Time-dependent and regional expression of GABA transporter mRNAs following amygdala-kindled seizures in rats. Molecular Brain Research, 54(1), 49-55. https://doi.org/10.1016/S0169-328X(97)00323-9