Time dependent amelioration against ischemic brain damage by glial cell line-derived neurotrophic factor after transient middle cerebral artery occlusion in rat

W. R. Zhang, T. Hayashi, M. Iwai, I. Nagano, K. Sato, Y. Manabe, Koji Abe

Research output: Contribution to journalArticle

47 Citations (Scopus)

Abstract

Time dependent influence of glial cell line-derived neurotrophic factor (GDNF) was examined after 90 min of transient middle cerebral artery occlusion (MCAO) in rats. Treatment with GDNF significantly reduced the infarct volume stained with 2,3,5-triphenyltetrazolium chloride (TTC) when GDNF was topically applied at 0 and 1 h of reperfusion, but became insignificant at 3 h as compared to vehicle group. The protective effect of GDNF was closely related to the significant reduction of the number of terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL) positive cells as well as immunofluorescently positive cells for active forms of caspases, especially active caspase-3 but not -9. Thus, the present study showed that topical application of GDNF significantly reduced infarct size in a time-dependent manner, while the therapeutic time window was shorter than other chemical compounds such as an NMDA receptor antagonist (MK-801) and a free radical scavenger (alpha-phenyl-tert-butyl-nitrone, PBN). The effect of GDNF was stronger in suppressing active caspase-3 than active caspase-9.

Original languageEnglish
Pages (from-to)253-256
Number of pages4
JournalBrain Research
Volume903
Issue number1-2
DOIs
Publication statusPublished - Jun 8 2001

Fingerprint

Glial Cell Line-Derived Neurotrophic Factor
Middle Cerebral Artery Infarction
Brain
In Situ Nick-End Labeling
Caspase 3
Free Radical Scavengers
Dizocilpine Maleate
DNA Nucleotidylexotransferase
Caspase 9
Biotin
Caspases
N-Methyl-D-Aspartate Receptors
Reperfusion

Keywords

  • Caspase-3
  • Caspase-9
  • Glial cell line-derived neurotrophic factor (GDNF)
  • Ischemia
  • Terminal deoxynucleotidyl transferase-mediated dUTP-biotin in situ nick end labeling (TUNEL)

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Time dependent amelioration against ischemic brain damage by glial cell line-derived neurotrophic factor after transient middle cerebral artery occlusion in rat. / Zhang, W. R.; Hayashi, T.; Iwai, M.; Nagano, I.; Sato, K.; Manabe, Y.; Abe, Koji.

In: Brain Research, Vol. 903, No. 1-2, 08.06.2001, p. 253-256.

Research output: Contribution to journalArticle

Zhang, W. R. ; Hayashi, T. ; Iwai, M. ; Nagano, I. ; Sato, K. ; Manabe, Y. ; Abe, Koji. / Time dependent amelioration against ischemic brain damage by glial cell line-derived neurotrophic factor after transient middle cerebral artery occlusion in rat. In: Brain Research. 2001 ; Vol. 903, No. 1-2. pp. 253-256.
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