Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique

Shigeki Ono, Isao Date, Keisuke Onoda, Takashi Ohmoto

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

In this report, we examined whether corrosion cast method is also applicable for the measurement and estimation of the rat major arteries in which subarachnoid hemorrhage (SAH) is produced. Additionally, we have examined the diameters of the rat major arteries following SAH. A total of 0.3 ml autologous blood was injected into the cisterna magna of male Sprague-Dawley rats for the SAH model. A perfusion of a semi-polymerized casting medium was performed, 10 min, 30 min, 1 h, 4 h, 8 h, 1 day, 2 days, 3 days, 5 days, and 7 days after SAH. The brains were corroded in a 10% NaOH solution. The BA and the other major arteries were then measured using scanning electron microscopy (SEM). Macroscopic observation and hematoxylin-eosin (HE) staining were also performed. Using the corrosion cast method, the biphasic contractile response was observed in the BA; 8.3% and 11.6% contractions were observed 30 min and 1 day after SAH, respectively. In addition, there was almost no smooth muscle or adventitial thickening in the chronic stage. In contrast, the dilative response was observed in the internal carotid artery and middle cerebral artery 10 min after SAH. Macroscopic findings and HE staining revealed that the extensive basal subarachnoid hematoma had almost disappeared by day 2. These results indicate that in this model, the minimal spasm, which occurs one day after SAH, can be explained by the small capacity of the rat subarachnoid space and the rapid cerebrospinal fluid washout around major vessels at the cerebral base. Moreover, the present data also show the compensatory dilatation in the ICA and MCA in the early stage after SAH.

Original languageEnglish
Pages (from-to)383-389
Number of pages7
JournalNeurological Research
Volume25
Issue number4
DOIs
Publication statusPublished - Jun 2003

Fingerprint

Cerebral Arteries
Corrosion
Subarachnoid Hemorrhage
Arteries
Hematoxylin
Eosine Yellowish-(YS)
Staining and Labeling
Cisterna Magna
Adventitia
Subarachnoid Space
Middle Cerebral Artery
Spasm
Internal Carotid Artery
Hematoma
Electron Scanning Microscopy
Smooth Muscle
Sprague Dawley Rats
Cerebrospinal Fluid
Dilatation
Perfusion

Keywords

  • Cerebral arteries
  • Corrosion cast technique
  • Subarachnoid hemorrhage
  • Vasospasm

ASJC Scopus subject areas

  • Clinical Neurology
  • Neuroscience(all)

Cite this

Time course of the diameter of the major cerebral arteries after subarachnoid hemorrhage using corrosion cast technique. / Ono, Shigeki; Date, Isao; Onoda, Keisuke; Ohmoto, Takashi.

In: Neurological Research, Vol. 25, No. 4, 06.2003, p. 383-389.

Research output: Contribution to journalArticle

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