Time-course effects of a single administration of cocaine on receptor binding and subunit mRNAs of GABA(A) receptors

Mika Yamaguchi, Toshihito Suzuki, Shuzo Abe, Atsuomi Baba, Takehiko Ito, Nobuo Okado

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

We investigated the time-course effects of a single administration of cocaine (20 mg/kg) on GABA(A) receptor binding labeled by t- [35S]butylbicyclophophorothionate (TBPS) and on several types of GABA(A) receptor subunit mRNAs in the rat brain by in vitro quantitative receptor autoradiography and in situ hybridization. The levels of α1, β2, and β3 subunit mRNAs in several brain regions such as the cortex, cerebellum, and striatum were significantly decreased within 1 h, while β3 subunit mRNA was increased in the dentate gyrus. All of these changes were transient, occurring within 1 h after the injection of cocaine. In the cortex and cerebellum, the reduction in α1 subunit mRNA was followed by a significant decrease in [35S]TBPS receptor binding, which occurred 4 h after cocaine injection. These findings suggest that acute cocaine administration discretely regulates GABA(A) receptor subunit mRNA levels in several brain regions through a change in transcription or turnover rates of subunit mRNAs, which may be closely related to cocaine-induced behavioral abnormalities. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)155-163
Number of pages9
JournalMolecular Brain Research
Volume81
Issue number1-2
DOIs
Publication statusPublished - Sep 30 2000

Keywords

  • Cocaine
  • GABA(A) receptor
  • Subunit mRNA
  • Time course
  • [S]TBPS binding

ASJC Scopus subject areas

  • Molecular Biology
  • Cellular and Molecular Neuroscience

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