TY - JOUR
T1 - Thymidylate synthase and dihydropyrimidine dehydrogenase mRNA levels in tumor tissues and the efficacy of 5-fluorouracil in patients with non-small-cell lung cancer
AU - Shintani, Yasushi
AU - Ohta, Mitsunori
AU - Hirabayashi, Hirohisa
AU - Tanaka, Hisaichi
AU - Iuchi, Keiji
AU - Nakagawa, Katsuhiro
AU - Maeda, Hajime
AU - Kido, Tetsuo
AU - Miyoshi, Shinichiro
AU - Matsuda, Hikaru
PY - 2004/8
Y1 - 2004/8
N2 - We examined 116 stage I-IIIA non-small-cell lung cancer (NSCLC) patients for intra-tumoral expression of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) using TaqMan reverse transcription polymerase chain reaction (RT-PCR) assay to clarify the correlation between gene expression and the efficacy of 5-fluorouracil (5-FU) in patients with NSCLC. Patients who were administered 5-FU alone after surgery comprised the 5-FU group (n=30), and those who underwent only surgery comprised the control group (n=86). When dichotomized at the mean TS and DPD mRNA level, patients with low-DPD tumors who were administered 5-FU had a significantly better prognosis than those who did not receive adjuvant treatment (p=0.041). In addition, in the 5-FU group, 10 patients with both low-TS and low-DPD tumors have not had any relapse, whereas 8 of the 20 patients with either high-TS or high-DPD tumors developed distant metastasis after surgery. Based on these results, the quantitation of TS and DPD mRNA levels may predict the efficacy of 5-FU after surgery for patient with NSCLC.
AB - We examined 116 stage I-IIIA non-small-cell lung cancer (NSCLC) patients for intra-tumoral expression of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) using TaqMan reverse transcription polymerase chain reaction (RT-PCR) assay to clarify the correlation between gene expression and the efficacy of 5-fluorouracil (5-FU) in patients with NSCLC. Patients who were administered 5-FU alone after surgery comprised the 5-FU group (n=30), and those who underwent only surgery comprised the control group (n=86). When dichotomized at the mean TS and DPD mRNA level, patients with low-DPD tumors who were administered 5-FU had a significantly better prognosis than those who did not receive adjuvant treatment (p=0.041). In addition, in the 5-FU group, 10 patients with both low-TS and low-DPD tumors have not had any relapse, whereas 8 of the 20 patients with either high-TS or high-DPD tumors developed distant metastasis after surgery. Based on these results, the quantitation of TS and DPD mRNA levels may predict the efficacy of 5-FU after surgery for patient with NSCLC.
KW - 5-Fluorouracil
KW - Dihydropyrimidine dehydrogenase
KW - Disease-free survival
KW - Non-small-cell lung cancer
KW - Real-time RT-PCR
KW - Thymidylate synthase
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UR - http://www.scopus.com/inward/citedby.url?scp=3042756923&partnerID=8YFLogxK
U2 - 10.1016/j.lungcan.2004.01.015
DO - 10.1016/j.lungcan.2004.01.015
M3 - Article
C2 - 15246190
AN - SCOPUS:3042756923
VL - 45
SP - 189
EP - 196
JO - Lung Cancer
JF - Lung Cancer
SN - 0169-5002
IS - 2
ER -