Thymidylate synthase and dihydropyrimidine dehydrogenase mRNA levels in tumor tissues and the efficacy of 5-fluorouracil in patients with non-small-cell lung cancer

Yasushi Shintani, Mitsunori Ohta, Hirohisa Hirabayashi, Hisaichi Tanaka, Keiji Iuchi, Katsuhiro Nakagawa, Hajime Maeda, Tetsuo Kido, Shinichiro Miyoshi, Hikaru Matsuda

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39 Citations (Scopus)

Abstract

We examined 116 stage I-IIIA non-small-cell lung cancer (NSCLC) patients for intra-tumoral expression of thymidylate synthase (TS) and dihydropyrimidine dehydrogenase (DPD) using TaqMan reverse transcription polymerase chain reaction (RT-PCR) assay to clarify the correlation between gene expression and the efficacy of 5-fluorouracil (5-FU) in patients with NSCLC. Patients who were administered 5-FU alone after surgery comprised the 5-FU group (n=30), and those who underwent only surgery comprised the control group (n=86). When dichotomized at the mean TS and DPD mRNA level, patients with low-DPD tumors who were administered 5-FU had a significantly better prognosis than those who did not receive adjuvant treatment (p=0.041). In addition, in the 5-FU group, 10 patients with both low-TS and low-DPD tumors have not had any relapse, whereas 8 of the 20 patients with either high-TS or high-DPD tumors developed distant metastasis after surgery. Based on these results, the quantitation of TS and DPD mRNA levels may predict the efficacy of 5-FU after surgery for patient with NSCLC.

Original languageEnglish
Pages (from-to)189-196
Number of pages8
JournalLung Cancer
Volume45
Issue number2
DOIs
Publication statusPublished - Aug 2004

Keywords

  • 5-Fluorouracil
  • Dihydropyrimidine dehydrogenase
  • Disease-free survival
  • Non-small-cell lung cancer
  • Real-time RT-PCR
  • Thymidylate synthase

ASJC Scopus subject areas

  • Oncology
  • Pulmonary and Respiratory Medicine
  • Cancer Research

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    Shintani, Y., Ohta, M., Hirabayashi, H., Tanaka, H., Iuchi, K., Nakagawa, K., Maeda, H., Kido, T., Miyoshi, S., & Matsuda, H. (2004). Thymidylate synthase and dihydropyrimidine dehydrogenase mRNA levels in tumor tissues and the efficacy of 5-fluorouracil in patients with non-small-cell lung cancer. Lung Cancer, 45(2), 189-196. https://doi.org/10.1016/j.lungcan.2004.01.015