Thymidine phosphorylase and dihydropyrimidine dehydrogenase expression levels in tumor and normal tissue specimens of T3 human colorectal carcinoma

Atsushi Okita, Kazunori Tsukuda, Masakazu Murakami, Tetsuya Ota, Hiroyoshi Doihara, Manabu Suda, Tomoharu Nakano, Kinya Matsuoka, Eiji Suzuki, Minoru Naito, Akio Andou, Nobuyoshi Shimizu

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3 Citations (Scopus)

Abstract

Purpose. Thymidine phosphorylase (TP) and dihydropyrimidine dehydrogenase (DPD) are important enzymes related to the metabolism of 5-fluorouracil and its derivatives. We evaluated the association between the clinicopathological factors and these enzymes in patients with T3 colorectal carcinoma. Methods. The TP and DPD expression levels in 15 patients with T3 colorectal carcinomas were measured in tumor and adjacent normal tissue specimens by enzyme-linked immunosorbent assay. Correlations between each enzyme and clinicopathological factors were also statistically evaluated. Results. The TP levels in tumor and normal tissue specimens were 77.9 ± 33.6 and 24.7 ± 10.3, respectively (P < 0.001). The DPD levels in tumor and normal tissue specimens were 44.1 ± 18.2 and 53.1 ± 24.1, respectively (P = 0.46). The TP/DPD ratios in tumor and normal tissue specimens were 1.84 ± 0.52 and 0.53 ± 0.26, respectively (P < 0.001). The tumor/normal ratios of TP level in patients with and without liver metastasis were 1.79 ± 0.91 and 4.67 ± 2.51, respectively (P = 0.024). Conclusion. The measurement of the enzyme expression levels of TP and DPD is considered to be useful for better understanding the conditions of tumor progression. The mechanisms of regulation of these enzymes thus require further evaluation.

Original languageEnglish
Pages (from-to)348-353
Number of pages6
JournalSurgery today
Volume36
Issue number4
DOIs
Publication statusPublished - Apr 2006

Keywords

  • Dihydropyrimidine dehydrogenase
  • T3 colorectal carcinoma
  • Thymidine phosphorylase

ASJC Scopus subject areas

  • Surgery

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    Okita, A., Tsukuda, K., Murakami, M., Ota, T., Doihara, H., Suda, M., Nakano, T., Matsuoka, K., Suzuki, E., Naito, M., Andou, A., & Shimizu, N. (2006). Thymidine phosphorylase and dihydropyrimidine dehydrogenase expression levels in tumor and normal tissue specimens of T3 human colorectal carcinoma. Surgery today, 36(4), 348-353. https://doi.org/10.1007/s00595-005-3145-x